Search
CREST-BRG1 complex
Function:
- regulates promoter activation by orchestrating Ca+2-dependent release of a repressor complex & a recruitment of an activator complex
- in resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex
- upon Ca+2 influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex
- at the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation
- the CREST-BRG1 complex also binds to the NR2B promoter, & activity-dependent induction of NR2B expression involves a release of HDAC1 & recruitment of CREBBP
Structure:
- subunits: SMARCA4/BRG1/BAF190A, SS18L1/CREST, HDAC1, RB1 & SP1
General
molecular complex
Properties
SUBUNITS: transcription activator BRG1
COMPARTMENT: cell nucleus
MOTIF: SS18L1/CREST interaction {1-282}
MOTIF: Thr phosphorylation site {T11}
HSA {460-532}
lysine-rich region {578-588}
MOTIF: lysine residue (SEVERAL)
Thr phosphorylation site {T609}
Ser phosphorylation site {S610}
Ser phosphorylation site {S613}
Ser phosphorylation site {S655}
Ser phosphorylation site {S657}
Ser phosphorylation site {S660}
Ser phosphorylation site {S662}
glutamate-rich region {663-672}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S695}
Ser phosphorylation site {S699}
Ser phosphorylation site {S721}
helicase
NAME: helicase
SITE: 766-931
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 779-786
DEAD/H box
NAME: DEAD/H box
SITE: 881-884
Helicase C-terminal {1084-1246}
glutamate-rich region {1360-1364}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S1382}
Ser phosphorylation site {S1452}
bromodomain {1477-1547}
MOTIF: peptide motif {1539-1540}
Ser phosphorylation site {S1570}
glutamate-rich region {1571-1584}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S1575}
Ser phosphorylation site {S1586}
Ser phosphorylation site {S1627}
Ser phosphorylation site {S1631}
Ser phosphorylation site {S1640}
Ser phosphorylation site {S1642}
Ser phosphorylation site {S1644}
SS18-like protein 1
MOTIF: glutamine-rich region {190-396}
MOTIF: glutamine residue (SEVERAL)
SH3-binding site
NAME: SH3-binding site
SITE: 371-379
histone deacetylase 1
COMPARTMENT: cell nucleus
MOTIF: Histone deacetylase {9-321}
MOTIF: histidine residue {H141}
Tyr phosphorylation site {Y221}
Ser phosphorylation site {S393}
Ser phosphorylation site {S421}
Ser phosphorylation site {S423}
binding site
FOR-BINDING-OF: retinoblastoma-associated protein
retinoblastoma-associated protein
COMPARTMENT: cell nucleus
MOTIF: alanine-rich region {10-18}
MOTIF: alanine residue (SEVERAL)
proline-rich region
SITE: 20-29
MOTIF: proline residue (SEVERAL)
binding site
SITE: N-TERMINAL
FOR-BINDING-OF: death domain protein p84
Ser phosphorylation site {S249}
FOR-PHOSPHORYLATION-BY: cyclin-dependent kinase-1
Thr phosphorylation site {T252}
FOR-PHOSPHORYLATION-BY: cyclin-dependent kinase-1
Thr phosphorylation site {T356}
Thr phosphorylation site {T373}
FOR-PHOSPHORYLATION-BY: cyclin-dependent kinase-1
Pocket
NAME: Pocket
SITE: 373-771
FOR-BINDING-OF: nucleolar transcription factor 1
SV40 T antigen
adenovirus E1a
E2F
histone deacetylase 1
MOTIF: domain A {373-579}
spacer {580-639}
domain B {640-771}
Ser phosphorylation site {S807}
FOR-PHOSPHORYLATION-BY: cyclin-dependent kinase
Ser phosphorylation site {S811}
FOR-PHOSPHORYLATION-BY: cyclin-dependent kinase
Thr phosphorylation site {T826}
transcription factor Sp1
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: Repressor {1-82}
MOTIF: Ser phosphorylation site {S7}
threonine-rich region {36-143}
MOTIF: threonine residue (SEVERAL)
Ser phosphorylation site {S59}
proteolytic site {63-64}
Ser phosphorylation site {S101}
transcriptional activation domain
SITE: 146-251
transcriptional activation domain
SITE: 261-495
MOTIF: threonine-rich region {271-379}
MOTIF: threonine residue (SEVERAL)
Thr phosphorylation site {T278}
Thr phosphorylation site {T453}
Ser glycosylation site {S491}
transcriptional activation domain
SITE: 496-610
Ser phosphorylation site {S612}
Ser glycosylation site {S612}
binding site
SITE: 619-785
FOR-BINDING-OF: Varicella zoster
MOTIF: Zn finger C2H2-type
SITE: 626-650
EFFECTOR-BOUND: Zn+2
Thr phosphorylation site {T640}
Thr glycosylation site {T640}
Ser phosphorylation site {S641}
Ser glycosylation site {S641}
Thr phosphorylation site {T651}
Zn finger C2H2-type
SITE: 656-680
EFFECTOR-BOUND: Zn+2
Thr phosphorylation site {T668}
Ser phosphorylation site {S670}
Thr phosphorylation site {T681}
Zn finger C2H2-type
SITE: 686-708
EFFECTOR-BOUND: Zn+2
Ser glycosylation site {S698}
Ser phosphorylation site {S702}
Ser glycosylation site {S702}
Domain D {708-785}
Thr phosphorylation site {T739}
References
UniProt :accession P51532
Components
histone deacetylase 1 (reduced potassium dependency 3 [RPD3] homolog-like 1, HDAC1, RPD3L1)
retinoblastoma-associated protein (pRb, Rb, pp110, p105-Rb, p110Rb, RB1)
SS18-like protein 1 (SYT homolog 1, SS18L1, KIAA0693)
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; transcription activator BRG1; ATP-dependent helicase SMARCA4; SNF2-beta; protein BRG-1; mitotic growth & transcription activator; protein brahma homolog 1; BRG1-associated factor 190A; BAF190A (SMARCA4, BAF190A, BRG1, SNF2B, SNF2L4)
transcription factor Sp1 (SP1, TSFP1)