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cohesion complex
Function:
1) complex required for cohesion of sister chromatids after DNA replication
2) forms a large proteinaceous ring within which sister chromatids can be trapped
3) at anaphase, complex is cleaved & dissociates from chromatin, allowing sister chromatids to segregate.
4) spindle pole assembly during mitosis
5) plays a role in apoptosis
- RAD21 subunit is cleaved by caspase-3 or caspase-7 early
- 64 kD RAD21 C-terminal cleavage product translocates to cytoplasm Stucture:
1) subunits
- SMC1L1 or SMC1L2
- SMC3
- RAD21
- STAG1, STAG2 or STAG3 (mutually exclusive)
2) cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) & SMC3 heterodimer attached via their hinge domain, RAD21 which link them, & one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21
Compartment:
- before prophase, it is scattered along chromosome arms
- during prophase, most of cohesin complexes dissociate from chromatin after phosphorylation by PLK, except at centromeres, where cohesin complexes remain
- at anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation
Related
cohesin complex (GO:0008278)
General
molecular complex
nuclear protein
Properties
COMPARTMENT: cell nucleus
SUBUNITS: stromal antigen
double-strand-break repair protein rad21 homolog
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: peptide motif {172-173}
peptide motif {279-280}
peptide motif {450-451}
proline-rich region
SITE: 469-510
MOTIF: proline residue (SEVERAL)
Structural maintenance of chromosome 3
COMPARTMENT: cell nucleus
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 32-39
coiled coil {179-350}
coiled coil {393-503}
coiled coil {669-916}
MOTIF: Ser phosphorylation site {S787}
coiled coil {958-989}
Ser phosphorylation site {S1013}
Ser phosphorylation site {S1065}
Ser phosphorylation site {S1067}
Ser phosphorylation site {S1074}
Ser phosphorylation site {S1083}
structural maintenance of chromosome 1-like 1 protein
COMPARTMENT: cell nucleus
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 32-39
coiled coil {104-124}
coiled coil {163-503}
MOTIF: Ser phosphorylation site {S360}
coiled coil {660-935}
Ser phosphorylation site {S957}
Ser phosphorylation site {S962}
Ser phosphorylation site {S966}
coiled coil {991-1068}
References
- UniProt :accession Q99496
- UniProt :accession O60216
Components
double-strand-break repair protein rad21 homolog (hHR2, nuclear matrix protein 1, NXP-1, SCC1 homolog, RAD21, HR21, KIAA0078, NXP1)
stromal antigen (STAG)
structural maintenance of chromosome 1-like 1 protein; SMC1-alpha protein (SMC1A, Sb1.8, SMC1L1, DXS423E, KIAA0178, SB18, SMC1)
Structural maintenance of chromosome 3 (chondroitin sulfate proteoglycan 6, chromosome-associated polypeptide, hCAP, bamacan, basement membrane-associated chondroitin proteoglycan, CSPG6, BAM, BMH, SMC3, SMC3L1)