CLIP-associating protein 1; cytoplasmic linker-associated protein 1; multiple asters homolog 1; protein Orbit homolog 1; hOrbit1 (CLASP1, KIAA0622, MAST1)

Function: - regulation of microtubule dynamics (density, length distribution & stability of interphase microtubles - participates in generating polarized microtubule networks - component of kinetochore outer corona region - mediates interactions between microtubule plus ends & cell cortex - microtubule plus-end tracking protein - promotes stabilization of dynamic microtubules - role in nucleation of noncentrosomal microtubules originating from the trans-Golgi network - required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell - may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 & PHLDB2 - this cortical microtubule stabilizing activity is regulated, at least in part, by phosphatidylinositol 3-kinase signaling - also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle - phosphorylated upon DNA damage, probably by ATM or ATR - interacts with CLIP2, ERC1, MAPRE1, MAPRE3, microtubules, PHLDB2 & RSN - interaction with ERC1 may be mediated by PHLDB2 - interacts with GCC2; - recruits CLASP1 to Golgi membranes - interacts with MACF1 (putative) Structure: - belongs to the CLASP family - contains 7 HEAT repeats Compartment: - cytoplasm, cytoskeleton, centrosome - chromosome, centromere, kinetochore, spindle - Golgi apparatus, trans-Golgi network - localizes preferentially near plus ends of growing spindle microtubules - localizes to centrosomes, kinetochores & the mitotic spindle from prometaphase - subsequently localizes to the spindle midzone from anaphase & to the midbody from telophase - in migrating cells, localizes to the plus ends of microtubules within the cell body & to the entire microtubule lattice within the lamella - localizes to the cell cortex & this requires ERC1 & PHLDB2

General

cytoskeletal protein HEAT repeat-containing protein (HEATR) phosphoprotein

Properties

SIZE: entity length = 1538 aa MW = 169 kD COMPARTMENT: cytoplasm golgi MOTIF: HEAT repeat {87-124} HEAT repeat {163-200} HEAT repeat {405-440} HEAT repeat {441-477} serine-rich region {530-765} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S545} Ser phosphorylation site {S559} Ser phosphorylation site {S568} Ser phosphorylation site {S594} Ser phosphorylation site {S598} Ser phosphorylation site {S600} Ser phosphorylation site {S609} Ser phosphorylation site {S636} Ser phosphorylation site {S646} Ser phosphorylation site {S647} Ser phosphorylation site {S649} Thr phosphorylation site {T651} Thr phosphorylation site {T656} MAPRE1/MAPRE3 interaction {662-785} MOTIF: Ser phosphorylation site {S688} Ser phosphorylation site {S695} Thr phosphorylation site {T711} Ser phosphorylation site {S714} Ser phosphorylation site {S797} HEAT repeat {974-1011} Ser phosphorylation site {S1091} Thr phosphorylation site {T1099} Ser phosphorylation site {S1196} Ser phosphorylation site {S1223} CLIP2 interaction {1254-1538} MOTIF: PHLDB2/RSN interaction {1254-1538} kinetochore localization {1256-1538} coiled coil {1299-1330} HEAT repeat {1342-1379} HEAT repeat {1460-1497} serine-rich region {1526-1532} MOTIF: serine residue (SEVERAL)

Database Correlations

OMIM 605852 UniProt Q7Z460 PFAM correlations Entrez Gene 23332 Kegg hsa:23332

References

UniProt :accession Q7Z460