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cleavage stimulation factor 64 kD subunit; CSTF 64 kD subunit; CstF-64; CF-1 64 kD subunit (CSTF2)
Function:
- one of the multiple factors required for polyadenylation & 3'-end cleavage of mammalian pre-mRNAs
- this subunit is directly involved in the binding to pre-mRNAs (putative)
- phosphorylated upon DNA damage, probably by ATM or ATR
- component of the CSTF complex
- CSTF2 directly interacts with CSTF3, SYMPK & RPO2TC1
- interacts with HSF1 in heat-stressed cells
- interacts with FIP1L1
Structure: contains 1 RRM domain (RNA recognition motif)
Compartment: nucleus
Alternative splicing: named isoforms=2
Expression: up-regulated during the G0 to S phase transition
General
nuclear protein
phosphoprotein
protein subunit
RNA-binding protein
Properties
SIZE: entity length = 577 aa
MW = 61 kD
COMPARTMENT: cell nucleus
MOTIF: RNP motif
NAME: RNP motif
SITE: 16-94
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
NAME: ribonucleoprotein-1 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
ribonucleoprotein-2 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
CSTF3/SYMPK interaction {108-248}
MOTIF: Ser phosphorylation site {S154}
proline-rich region
SITE: 198-409
MOTIF: proline residue (SEVERAL)
M-E-A-R-[AG] repeats {410-469}
MOTIF: consensus repeat {410-414}
consensus repeat {415-419}
consensus repeat {420-424}
consensus repeat {425-429}
consensus repeat {430-434}
consensus repeat {435-439}
consensus repeat {440-444}
consensus repeat {445-449}
consensus repeat {450-454}
consensus repeat {455-459}
consensus repeat {460-464}
consensus repeat {465-469}
proline-rich region
SITE: 470-526
MOTIF: proline residue (SEVERAL)
RPO2TC1 interaction {514-577}
MOTIF: Ser phosphorylation site {S518}
Ser phosphorylation site {S524}
Database Correlations
OMIM 600368
UniProt P33240
Pfam PF00076
Entrez Gene 1478
Kegg hsa:1478
References
- UniProt :accession P33240
- Kleiman FE & Manley JL
Functional interaction of BRCA1-associated BARD1 with
polyadenylation factor CstF-50
Science 285:1576-9 1999
PMID: 10477523
Component-of
pre-mRNA cleavage complex 2 (pre mRNA 3' end processing complex)