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cleavage stimulation factor 50 kD subunit; CSTF 50 kD subunit; CF-1 50 kD subunit; CstF-50 (CSTF1)
Function:
- one of the multiple factors required for polyadenylation & 3'-end cleavage of mammalian pre-mRNAs
- may be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA
- component of the CSTF complex
- interacts directly with CSTF3
- interacts with BARD1
Structure:
- the N-terminus is blocked
- homodimer
- N-terminus mediates homodimerization
- contains 6 WD repeats
- the WD6 domain is required for interaction with BARD1
- WD domains are responsible for interaction with CSTF3
Compartment: nucleus
General
nuclear protein
phosphoprotein
protein subunit
RNA-binding protein
WD repeat protein family
Properties
SIZE: entity length = 431 aa
MW = 48 kD
COMPARTMENT: cell nucleus
MOTIF: Hydrophobic {14-35}
WD repeat {106-145}
WD repeat {171-210}
WD repeat {215-254}
WD repeat {260-301}
WD repeat {303-343}
Tyr phosphorylation site {Y367}
WD repeat {395-430}
Database Correlations
OMIM 600369
UniProt Q05048
Pfam PF00400
Entrez Gene 1477
Kegg hsa:1477
References
- UniProt :accession Q05048
- Kleiman FE & Manley JL
Functional interaction of BRCA1-associated BARD1 with
polyadenylation factor CstF-50
Science 285:1576-9 1999
PMID: 10477523
Component-of
cleavage stimulation factor