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class B basic helix-loop-helix protein 8; bHLHB8; muscle, intestine & stomach expression 1; MIST-1 (BHLHB8, MIST1, BHLHA15)

Function: 1) role in controlling transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated 2) transcriptional repression via muscle-specific E-box occupancy by homodimers 3) negatively regulates bHLH-mediated transcription through an N-terminal repressor domain (putative) 4) regulator of acinar cell function, stability, & identity 5) required for normal organelle localization in exocrine cells & for mitochondrial Ca+2 transport 6) regulator of gene expression in several different embryonic & postnatal cell lineages 7) binds to the E-box consensus sequence 5'-CANNTG-3' 8) forms homodimers or heterodimers with TCF3 gene products E12 & E47 - these dimers bind to the E-box site 9) heterodimer with MYOD1 does NOT bind target DNA Structure: 1) lacks a classic transcription activation domain 2) N-terminal region capable of inhibiting heterologous activators 3) contains 1 basic helix-loop-helix (bHLH) domain Compartment: nucleus Expression: - expressed in brain, liver, spleen, skeletal muscle

General

DNA-binding protein

Properties

SIZE: MW = 21 kD entity length = 189 aa COMPARTMENT: cell nucleus MOTIF: DNA-binding motif SITE: 57-77 helix-loop-helix NAME: helix-loop-helix SITE: 78-128 FOR-BINDING-VIA: helix-loop-helix

Database Correlations

OMIM 608606 UniProt Q7RTS1 Pfam PF00010

References

UniProt :accession Q7RTS1