chloride transport protein 6; chloride channel protein 6; ClC-6 (CLCN6 KIAA0046)

Function: - chloride transport protein - initially identified as voltage-gated chloride channel - the presence of the conserved gating Glu suggests that it functions as antiporter Structure: - N-glycosylated on several asparagine residues - belongs to the chloride channel (TC 2.A.49) family - contains 2 CBS domains Compartment: - endosome membrane; multi-pass membrane protein - detected in detergent-resistant lipid rafts Alternative splicing: named isoforms=5; A-E Expression: - testis, ovary, small intestine, brain & skeletal muscle - low level expression in aortic & coronary vascular smooth muscle cells, & aortic endothelial cells - isoform C is only detected in kidney Notes: - the CLC channel family contains both chloride channels & proton-coupled anion transporters that exchange chloride or another anion for protons - the presence of conserved gating Glu is typical for family members that function as antiporters (putative)

General

Cl- channel glycoprotein transmembrane 10 protein

Properties

SIZE: entity length = 869 aa MW = 97 kD COMPARTMENT: plasma membrane MOTIF: cytoplasmic domain {1-80} MOTIF: cysteine-rich region {4-18} transmembrane domain {81-113} exoplasmic loop {114-127} transmembrane domain {128-150} cytoplasmic loop {151-175} MOTIF: peptide motif {156-160} binding site SITE: 157-157 FOR-BINDING-OF: Chloride In-membrane helix {159-166} transmembrane domain {176-194} exoplasmic loop {195-199} peptide motif {198-202} transmembrane domain {200-217} MOTIF: glutamate residue {200} cytoplasmic loop {218-276} MOTIF: In-membrane helix {241-253} In-membrane helix {257-265} glutamate residue {267} transmembrane domain {277-294} exoplasmic loop {295-334} transmembrane domain {335-364} cytoplasmic loop {365-370} transmembrane domain {371-392} exoplasmic loop {393-461} MOTIF: N-glycosylation site {N410} N-glycosylation site {N422} N-glycosylation site {N432} transmembrane domain {462-481} cytoplasmic loop {482-486} transmembrane domain {487-511} MOTIF: peptide motif {487-491} binding site SITE: 489-489 FOR-BINDING-OF: Cl- exoplasmic loop {512-552} MOTIF: In-membrane helix {519-533} In-membrane loop {534-536} In-membrane helix {537-548} In-membrane loop {549-552} transmembrane domain {553-571} cytoplasmic domain {572-869} MOTIF: binding site SITE: 576-576 FOR-BINDING-OF: Chloride CBS 1 {605-662} ATP-binding site NAME: ATP-binding site SITE: 630-632 CBS 2 {807-868} ATP-binding site NAME: ATP-binding site SITE: 849-852 ION-PERMEABILITY: Cl-

References

UniProt :accession P51797