chordin-like protein 2 (chordin-related protein 2, breast tumor novel factor 1, BNF-1, CHRDL2, BNF1, CHL2, UNQ765/PRO1557)

Function: - may inhibits BMPs activity by blocking their interaction with their receptors - negative regulator effect on cartilage formation/regeneration from immature mesenchymal cells, by preventing or diminidhing the rate of matrix accumulation - role during myoblast & osteoblast differentiation, & maturation - interacts with GDF5 - may interact with BMP2, BMP4, BMP5, BMP6, BMP7 & INHBA Structure: - contains 3 VWFC domains Compartment: - isoforms 1 & 2: secreted - isoforms 3, 4 & 5: cytoplasm Alternative splicing: named isoforms=5; 5 additional mRNAs also exist Expression: - expressed in uterus > heart, liver, prostate, testis, ovary > skeletal muscle, kidney, spleen, small intestine, colon - expressed in the secretory epithelial cells of uterine endometrium, fallopian tubes, endocervical glands, bladder & prostate - expressed in transitional epithelium of urinary bladder - expressed in bone osteoblasts (at protein level) - differential expression of isoforms observed during myoblast & osteoblast differentiation & maturation - in normal cartilage, expression is confined in a few chondrocytes in the superficial zone as well as in the middle zone - isoform 1 & 2 are expressed in fetal cerebellum & heart - only isoform 2 is detected in fetal spleen Pathology: - role in tumor angiogenesis - in diseased cartilage coming from osteoarthritic patients, expression was limited to the middle zone of chondrocytes - up-regulated in breast cancer, lung cancer & colon cancer

General

glycoprotein secreted protein

Properties

SIZE: MW = 47 kD entity length = 429 aa COMPARTMENT: cytoplasm MOTIF: signal sequence {1-25} VWFC domain {31-96} VWFC domain {109-175} MOTIF: N-glycosylation site {N114} VWFC domain {250-315}

Database Correlations

UniProt Q6WN34 Pfam PF00093

References

UniProt :accession Q6WN34