choline/ethanolaminephosphotransferase 1 (hCEPT1, CEPT1)

Function: - catalyzes both phosphatidylcholine & phosphatidylethanolamine biosynthesis from CDP-choline & CDP-ethanolamine, respectively - role in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface - higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity CDP-ethanolamine + 1,2-diacylglycerol CMP + phosphatidylethanolamine CDP-choline + 1,2-diacylglycerol CMP + phosphatidylcholine Cofactor: Mg⁺², Mn+2 Kinetic parameters: - KM=37 uM for CDP-choline - KM=101 uM for CDP-ethanolamine - Vmax=10.5 nmol/min/mg enzyme with CDP-choline as substrate - Vmax=4.35 nmol/min/mg enzyme with CDP-ethanolamine as substrate Structure: - belongs to the CDP-alcohol phosphatidyltransferase class-I family Compartment: - endoplasmic reticulum membrane - nuclear membrane Expression: ubiquitously expressed

General

glycoprotein phosphotransferase transmembrane 8 protein

Properties

SIZE: MW = 47 kD entity length = 416 aa COMPARTMENT: cell nucleus endoplasmic reticulum MOTIF: transmembrane domain {87-107} transmembrane domain {115-135} N-glycosylation site {N144} transmembrane domain {186-206} transmembrane domain {209-229} transmembrane domain {239-259} transmembrane domain {283-303} transmembrane domain {317-337} transmembrane domain {365-385}

Database Correlations

UniProt Q9Y6K0 Pfam PF01066 ENZYME correlations

References

UniProt :accession Q9Y6K0