chloride intracellular channel protein 4; intracellular chloride ion channel protein p64H1 (CLIC4)

Function: - can insert into membranes & form poorly selective ion channels that may also transport chloride ions - channel activity depends on the pH - membrane insertion seems to be redox-regulated & may occur only under oxidizing conditions - promotes cell-surface expression of HRH3 - has alternate cellular functions including a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis & cytokinesis - could also promote endothelial cell proliferation & regulate endothelial morphogenesis (tubulogenesis) - component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, IQGAP1 & CLIC5A - binds directly to brain dynamin 1 in a complex containing actin, tubulin & 14-3-3 isoforms - monomer - interacts with HRH3 - interacts with AKAP9 Structure: - members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane & functions as chloride channel - a conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion - belongs to the chloride channel CLIC family - contains 1 GST C-terminal domain Compartment: - cytoplasm, cytoskeleton, centrosome - cytoplasmic vesicle membrane - single-pass membrane protein (probable) - nuclear matrix - cell membrane - mitochondria - cell junction - colocalized with AKAP9 at the centrosome & midbody - exists both as soluble cytoplasmic protein & as membrane protein with probably a single transmembrane domain - present in an intracellular vesicular compartment that likely represents trans-Golgi network vesicles Expression: - detected in epithelial cells from colon, esophagus & kidney (at protein level) - expression is prominent in heart, kidney, placenta & skeletal muscle - up-regulated by Ca⁺² in differentiating keratinocytes - up-regulated in myofibroblasts

Properties

SIZE: entity length = 253 aa MW = 29 kD COMPARTMENT: mitochondria cytoplasm cell nucleus golgi MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl transmembrane domain {37-57} GST C-terminal {104-244}

Database Correlations

OMIM 606536 UniProt Q9Y696 Entrez Gene 25932 Kegg hsa:25932

References

UniProt :accession Q9Y696