cell division cycle-associated protein 2 (recruits PP1 onto mitotic chromatin at anaphase protein, Repo-Man, CDCA2)

Function: - regulator of chromosome structure during mitosis - required for condensin-depleted chromosomes to retain their compact architecture through anaphase. - acts by mediating recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase & into the following interphase - at anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates - interacts with PPP1CC - phosphorylated by CDK1 - may regulate its subcellular location Compartment: - nucleus - excluded from the nucleolus - present in nucleoplasm throughout the G1, S & G2 stages of the cell cycle - during M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, & faintly accumulates later on metaphase chromatin - as the cell progresses to anaphase, it accumulates on chromatin Alternative splicing: named isoforms=2 Expression: ubiquitously expressed

General

cell division cycle-associated protein 4 (CDCA) phosphoprotein

Properties

SIZE: MW = 113 kD entity length = 1024 aa COMPARTMENT: nuclear membrane MOTIF: Ser phosphorylation site {S292} Ser phosphorylation site {S336} PP1-binding {393-396} Ser phosphorylation site {S401} lysine-rich region {534-576} MOTIF: lysine residue (SEVERAL)

References

UniProt :accession Q69YH5