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CDK-activating kinase assembly factor MAT1; RING finger protein MAT1; menage a trois; CDK7/cyclin-H assembly factor; p36; p35; cyclin-G1-interacting protein; RING finger protein 66 (MNAT1, CAP35, MAT1, RNF66)
Function:
- stabilizes cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex
- CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 & CDK6 by Thr phosphorylation
- CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase 2 by Ser phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter & elongation of the transcripts
- involved in cell cycle control & in RNA transcription by RNA polymerase 2
- associates primarily with CDK7 & cyclin H to form the CAK complex
- CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor
Structure:
- contains 1 RING-type Zn+2 finger
- contains 1 UIM (ubiquitin-interacting motif) repeat
Compartment:
- nucleus
Expression:
- highest levels in colon & testis
- moderate levels are present thymus, prostate, ovary, & small intestine
- lowest levels are found in spleen & leukocytes
General
nuclear protein
phosphoprotein
ring finger protein
Properties
SIZE: entity length = 309 aa
MW = 36 kD
COMPARTMENT: cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
RING-finger {6-50}
EFFECTOR-BOUND: Zn+2
FOR-BINDING-OF: DNA motif
Thr phosphorylation site {T51}
UIM {142-161}
Ser phosphorylation site {S205}
Ser phosphorylation site {S279}
Database Correlations
OMIM 602659
UniProt P51948
Pfam PF06391
Entrez Gene 4331
Kegg hsa:4331
References
- UniProt :accession P51948
- NIEHS-SNPs
http://egp.gs.washington.edu/data/mnat1/
Component-of
CDK7/cyclin H complex, CDK-activating kinase (CAK) or p34cdc2 activating kinase