Cap methyltransferase 2; Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2; Cap2 2'O-ribose methyltransferase 2; HMTr2; MTr2; FtsJ methyltransferase domain-containing protein 1; protein adrift homolog (CMTR2 AFT FTSJD1)

Function: - S-adenosyl-L-methionine (SAM)-dependent methyltransferase - mediates mRNA cap2 2'-O-ribose methylation to the 5'mRNA-cap structure - methylates ribose of the second nucleotide of an m(7)GpppG-capped mRNA & small nuclear RNA (snRNA) (cap0) to produce m(7)GpppRmpNm (cap2) - recognizes a guanosine cap on RNA independently of its N(7) methylation status - displays cap2 methylation on both cap0 & cap1 - displays a preference for cap1 RNAs S-adenosyl-L-methionine + m(7)G(5')pppRmpN-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppRmpNm-RNA Structure: - contains 1 adrift-type SAM-dependent 2'-O-MTase domain Compartment: - nucleus, cytoplasm, membrane - single-pass membrane protein (putative)

General

membrane protein methyltransferase nuclear protein

Properties

SIZE: entity length = 770 aa MW = 88 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: SAM-dependent 2'-O-MTase {109-322} MOTIF: binding site SITE: 148-148 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 167-167 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 235-235 FOR-BINDING-OF: S-adenosylmethionine lysine residue {K275} transmembrane domain {642-662}

Database Correlations

UniProt Q8IYT2 Pfam PF01728 Entrez Gene 55783 Kegg hsa:55783

References

UniProt :accession Q8IYT2