Cap methyltransferase 1; Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1; Cap1 2'O-ribose methyltransferase 1; MTr1; hMTr1; FtsJ methyltransferase domain-containing protein 2; interferon-stimulated gene 95 kD protein; ISG95 (CMTR1, FTSJD2, KIAA0082, MTR1)

Function: - S-adenosyl-L-methionine-dependent methyltransferase - mediates mRNA cap1 2'-O-ribose methylation to 5'-cap structure of mRNAs - methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA & small nuclear RNA (snRNA) to produce m(7)GpppRm (cap1) - preference for cap0 transcripts - Cap1 modification is linked to higher levels of translation - may be involved in the interferon response pathway - interacts with POLR2A (via C-terminus) S-adenosyl-L-methionine + m(7)G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppRm-RNA Structure: - contains 1 G-patch domain - contains 1 RrmJ-type SAM-dependent 2'-O-MTase domain - contains 1 WW domain Compartment: nucleus Expression: - induced by interferons alpha & beta, & by vaccinia virus infection

General

methyltransferase nuclear protein phosphoprotein

Properties

SIZE: entity length = 835 aa MW = 95 kD COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S28} Ser phosphorylation site {S31} G-patch domain {87-133} RrmJ-type SAM-dependent 2'-O-MTase {231-450} MOTIF: binding site SITE: 281-281 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 311-311 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 364-364 FOR-BINDING-OF: S-adenosylmethionine lysine residue {K404} POLR2A interaction {727-835} MOTIF: WW domain (W/rsp5/WWP domain) {752-786}

Database Correlations

UniProt Q8N1G2 PFAM correlations Entrez Gene 23070 Kegg hsa:23070 ENZYME 2.1.1.57

References

UniProt :accession Q8N1G2