Ca+2-independent phospholipase A2-gamma (intracellular membrane-associated Ca+2-independent phospholipase A2 gamma, iPLA2-gamma, patatin-like phospholipase domain-containing protein 8, PNPLA8, IPLA22, IPLA2G, BM-043)

Function: - Ca⁺²-independent phospholipase A2 - catalyzes hydrolysis of sn-2 position of glycerophospholipids, PtdSer & to a lower extent PtdCho - cleaves membrane phospholipids - inhibited by E-6-bromomethylene-3-1-naphthalenyl-2H- tetrahydropyran-2-one (BEL) 2-lysophosphatidylcholine + H₂O glycerophosphocholine + carboxylate Kinetic parameters: - Optimum pH is 8.0 Structure: contains 1 patatin domain Compartment: - membrane (putative) - perinuclear, endoplasmic reticulum & Golgi Alternative splicing: named isoforms=2 Expression: - expressed in parenchymal tissues including heart, skeletal muscle, placenta, brain, liver & pancreas - also expressed in bronchial epithelial cells & kidney - highest expression is observed in skeletal muscle & heart

General

glycoprotein phospholipase A2; phosphatidylcholine 2-acylhydrolase (PLA2)

Properties

SIZE: entity length = 782 aa MW = 88 kD MOTIF: N-glycosylation site {N4} N-glycosylation site {N361} active site MOTIF: histidine residue cysteine residue (SEVERAL) aspartate residue cysteine-rich region Patatin {445-640} MOTIF: transmembrane domain {475-495} GXSXG {481-485}

Database Correlations

UniProt Q9NP80 Pfam PF01734

References

UniProt :accession Q9NP80