bifunctional protein NCOAT; meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase & acetyltransferase; includes: protein O-GlcNAcase; glycoside hydrolase O-GlcNAcase; hexosaminidase C; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; OGA; includes: histone acetyltransferase; HAT (MGEA5, HEXC, KIAA0679, MEA5)

Function: 1) hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides 2) cleaves GlcNAc but not GalNAc from glycopeptides 3) can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc 4) possesses hyaluronidase activity 5) acetylates Lys-8 of histone H4 & Lys-14 of histone H3 6) binds both acetylated & unacetylated histone H4 tail - acetylation on Lys-8 of histone H4 abolishes binding 7) inhibited by N-acetylglucosamine, but not N-acetylgalactosamine 8) proteolytically cleaved by caspase-3 Kinetic parameters: - KM=1.1 mM for pNP-GLcNAc - Vmax=652 umol/min/mg enzyme with pNP-GLcNAc as substrate - Optimum pH is 5.7-7 Structure: monomer Compartment: - isoform 1: cytoplasm - isoform 3: nucleus Alternative splicing: named isoforms=3 Expression: - ubiquitous - highest expression in brain, placenta, pancreas

Related

beta N-acetylhexosaminidase histone acetyltransferase

General

enzyme phosphoprotein

Properties

SIZE: entity length = 916 aa MW = 103 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: aspartate residue {D175} aspartate residue {D177} Ser phosphorylation site {S364} Histone acetyltransferase activity {583-916} MOTIF: histone H4 binding {695-814} cysteine residue {C777} MODIFICATION: cysteine residue {C793} cysteine residue {C793} MODIFICATION: cysteine residue {C777}

Database Correlations

OMIM 604039 UniProt O60502 Pfam PF07555 Entrez Gene 10724 Kegg hsa:10724 ENZYME correlations

References

UniProt :accession O60502