Search
beta-Ala-His dipeptidase (carnosine dipeptidase 1, CNDP dipeptidase 1, serum carnosinase, glutamate carboxypeptidase-like protein 2, CNDP1, CN1, CPGL2, UNQ1915/PRO4380)
Function:
- preferential hydrolysis of beta-Ala-|-His dipeptide (carnosine), & also anserine, Xaa-|-His dipeptides & other dipeptides including homocarnosine
Cofactor: binds 2 Zn+2
Inhibition:
- inhibited by the metal chelator 1,10-o-phenantrolin
- inhibitory concentration 50% (IC(50)) is 5 uM
Kinetic parameters:
- KM=1.27 uM for carnosine
- KM=11.00 uM for carnosine
- KM=0.20 uM for homocarnosine
- KM=1.0 uM for homocarnosine
- Optimum pH is 8.5
Structure:
- homodimer
- belongs to the peptidase M20A family
Compartment: secreted
Expression: expressed in adult nervous central system
Pathology:
- diabetic patients with the CNDP1 Mannheim allele are less susceptible to nephropathy.
- deficiency of CNDP1 may be the cause of homocarnosinosis
Polymorphism:
- number of trinucleotide (CTG) repeat varies among different alleles leading to insertion of Leu residues in signal peptide
- allele with 5 leucines is Mannheim allele
General
dipeptidase
glycoprotein
Properties
SIZE: MW = 57 kD
entity length = 507 aa
MOTIF: signal sequence {1-26}
Zn+2-binding site
SITE: 132-132
aspartate residue {D134}
Zn+2-binding site
SITE: 165-165
glutamate residue {E199}
Zn+2-binding site
SITE: 200-200
Zn+2-binding site
SITE: 228-228
N-glycosylation site {N322}
N-glycosylation site {N382}
Zn+2-binding site
SITE: 478-478
Database Correlations
UniProt Q96KN2
PFAM correlations
ENZYME 3.4.13.20
References
UniProt :accession Q96KN2