beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase, C2/4GnT, C2GnT-mucin type, C2GnT-M, hC2GnT-M, GCNT3)

Function: 1) protein glycosylation, mucin biosynthesis 2) glycosyltransferase, can synthesize all known mucin beta 6 N-acetylglucosaminides 3) mediates core 2 & core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis 4) I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce blood group I antigen during embryonic development UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)- N-acetyl-D-galactosaminyl-R Structure: - N-glycosylated - belongs to the glycosyltransferase 14 family Compartment: Golgi membrane Expression: - primarily expressed in mucus-secreting tissues - expressed in colon, kidney, small intestine, trachea, & stomach, where mucin is produced - induced by all-trans retinoic acid, TNF-alpha & IL13 Pathology: - strongly down-regulated in colorectal cancer

General

glycoprotein glycosyltransferase membrane protein

Properties

SIZE: MW = 51 kD entity length = 438 aa COMPARTMENT: golgi MOTIF: transmembrane domain {7-26} cysteine residue {C70} MODIFICATION: cysteine residue {C227} cysteine residue {C161} MODIFICATION: cysteine residue {C382} cysteine residue {C182} MODIFICATION: cysteine residue {C209} cysteine residue {C209} MODIFICATION: cysteine residue {C182} cysteine residue {C227} MODIFICATION: cysteine residue {C70} N-glycosylation site {N289} cysteine residue {C382} MODIFICATION: cysteine residue {C161} cysteine residue {C391} MODIFICATION: cysteine residue {C423} cysteine residue {C423} MODIFICATION: cysteine residue {C391}

Database Correlations

OMIM 606836 UniProt O95395 Pfam PF02485 ENZYME correlations

References

UniProt :accession O95395