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fibroblast growth factor 2; FGF-2; basic fibroblast growth factor; bFGF; heparin-binding growth factor 2; HBGF-2; prostatropin (FGF2, FGFB)

Function: - requires heparan sulfate binding for activity - heparin-binding growth factors are angiogenic agents in vivo & potent mitogens vitro - binds heparin more strongly than does aFGF - cell injury may liberate b-FGF - precursor does NOT contain ER signal sequence - role in regulation of cell survival, cell division, angiogenesis, cell differentiation & cell migration. - potent mitogen in vitro - phosphorylation at Tyr-215 regulates FGF2 unconventional secretion - interacts with FGFR1, FGFR2, FGFR3 & FGFR4 - affinity between fibroblast growth factors (FGFs) & their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors - interacts with CSPG4, FGFBP1 & TEC - found in a complex with FGFBP1, FGF1 & FGF2 Kinetic parameters: - pH dependence: retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degrees C - temperature dependence: inactivated after 3 minutes at 60 degrees celsius or 1 minute at 80 degrees C Structure: - monomer, homodimer - belongs to the heparin-binding growth factors family Compartment: - secreted - exported from cells by an endoplasmic reticulum (ER)/Golgi- independent mechanism - unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane - present in the cytoplasm of most cells Alternative initiation: Named isoforms=4 Expression: - expressed in granulosa & cumulus cells Pathology: - may play a role in resistanct to cancer chemotherapy [5] - expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue Notes: - several N-termini starting at positions 94, 125, 126, 132, 143 & 162 have been identified by direct sequencing

General

fibroblast growth factor phosphoprotein regulatory molecule secreted peptide without leader

Properties

SIZE: entity length = 288 aa MW = 31 kD COMPARTMENT: golgi endoplasmic reticulum MOTIF: binding site SITE: 169-169 FOR-BINDING-OF: Heparin Cell attachment site; atypical {179-181} Tyr phosphorylation site {Y215} Cell attachment site; atypical {221-223} binding site SITE: 261-277 FOR-BINDING-OF: heparin SECRETED-BY: most cell types*

Database Correlations

OMIM 134920 UniProt P09038 Pfam PF00167 Entrez Gene 2247 Kegg hsa:2247

References

  1. Machida CN et al NGF induction of the gene encoding the protease transin accompanies neuronal differentiation in PC12 cells. Neuron 1989 Jun;2(6):1587-96 PMID: 2560648 [
  2. Imamura T et al Recovery of mitogenic activity of a growth factor mutant with a nuclear translocation sequence. Science 249:1567 1990 PMID: 1699274
  3. Henderson B & Blake S Therapeutic potential of cytokine manipulation TIPS 13:145 1992 PMID: 1589908
  4. Entrez Gene :accession 2247
  5. Netscape, Dec 3, 2006 sites BBC News
  6. UniProt :accession P09038
  7. NIEHS-SNPs http://egp.gs.washington.edu/data/fgf2/