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A disintegrin & metalloproteinase domain 33 (ADAM33)
Function:
- precursor is cleaved by a furin endopeptidase
Cofactor: binds 1 Zn+2 per subunit (putative)
Structure:
- the conserved Cys present in the cysteine-switch motif binds the catalytic Zn+2, thus inhibiting the enzyme
- dissociation of Cys from Zn+2 upon activation- peptide release activates the enzyme
- contains 1 disintegrin domain
- contains 1 EGF-like domain
- contains 1 peptidase M12B domain
Compartment: membrane
Alternative splicing: named isoforms=3
Expression:
- expressed in all tissues, except liver, with high expression in placenta, lung, spleen & veins
Pathology:
- defects in ADAM33 may be a cause of susceptibility to asthma
General
ADAM (A disintegrin & metalloproteinase domain); MDC (metalloproteinase, disintegrin, cysteine-rich) protein
glycoprotein
phosphoprotein
Properties
SIZE: entity length = 813 aa
MW = 88 kD
COMPARTMENT: cellular membrane
MOTIF: signal sequence {1-29}
N-glycosylation site {N109}
Cysteine switch {131-138}
MOTIF: Zn+2-binding site
SITE: 133-133
N-glycosylation site {N145}
Peptidase M12B {210-409}
MOTIF: N-glycosylation site {N231}
Ser phosphorylation site {S269}
N-glycosylation site {N276}
cysteine residue {C320}
MODIFICATION: cysteine residue {C404}
Zn+2-binding site
SITE: 345-345
glutamate residue {E346}
Zn+2-binding site
SITE: 349-349
Zn+2-binding site
SITE: 355-355
cysteine residue {C360}
MODIFICATION: cysteine residue {C388}
cysteine residue {C361}
MODIFICATION: cysteine residue {C371}
cysteine residue {C371}
MODIFICATION: cysteine residue {C361}
cysteine residue {C388}
MODIFICATION: cysteine residue {C360}
cysteine residue {C404}
MODIFICATION: cysteine residue {C320}
disintegrin domain {417-503}
MOTIF: N-glycosylation site {N448}
cysteine residue {C475}
MODIFICATION: cysteine residue {C495}
cysteine residue {C495}
MODIFICATION: cysteine residue {C475}
cysteine-rich region {503-648}
EGF domain {649-681}
MOTIF: cysteine residue {C653}
MODIFICATION: cysteine residue {C663}
cysteine residue {C657}
MODIFICATION: cysteine residue {C669}
cysteine residue {C663}
MODIFICATION: cysteine residue {C653}
cysteine residue {C669}
MODIFICATION: cysteine residue {C657}
cysteine residue {C671}
MODIFICATION: cysteine residue {C680}
cysteine residue {C680}
MODIFICATION: cysteine residue {C671}
transmembrane domain {702-722}
Database Correlations
OMIM 607114
UniProt Q9BZ11
PFAM correlations
Entrez Gene 80332
Kegg hsa:80332
References
- UniProt :accession Q9BZ11
- NIEHS-SNPs
http://egp.gs.washington.edu/data/adam33/