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A disintegrin & metalloproteinase domain 28; metalloproteinase-like, disintegrin-like, & cysteine-rich protein-L; MDC-L; eMDC II (ADAM28, ADAM23, MDCL)
Function:
- may play a role in adhesive & proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL & CD40L
- may be involved in sperm maturation
- pro-domain removal & maturation may be, at least in part, autocatalytic
Cofactor: binds 1 Zn+2 per subunit (putative)
Structure:
- the conserved Cys present in the cysteine-switch motif binds the catalytic Zn+2, thus inhibiting the enzyme
- dissociation of Cys from Zn+2 upon activation- peptide release activates the enzyme
- contains 1 disintegrin domain
- contains 1 EGF-like domain
- contains 1 peptidase M12B domain
Compartment:
- isoform 1: cell membrane
- isoform 2: secreted
Alternative splicing: named isoforms=2
Expression:
- expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix & trachea
- the lymphocyte population is responsible for expression in these tissues
- isoform 2 is expressed preferentially in spleen
General
ADAM (A disintegrin & metalloproteinase domain); MDC (metalloproteinase, disintegrin, cysteine-rich) protein
glycoprotein
Properties
SIZE: MW = 87 kD
entity length = 775 aa
MOTIF: metalloprotease domain {199-399}
MOTIF: N-glycosylation site {N268}
N-glycosylation site {N275}
cysteine residue {C315}
MODIFICATION: cysteine residue {C394}
active site
SITE: E340
cysteine residue {C354}
MODIFICATION: cysteine residue {C378}
cysteine residue {C378}
MODIFICATION: cysteine residue {C354}
cysteine residue {C394}
MODIFICATION: cysteine residue {C315}
disintegrin domain {407-493}
MOTIF: cysteine residue {C465}
MODIFICATION: cysteine residue {C478}
cysteine residue {C478}
MODIFICATION: cysteine residue {C465}
cysteine-rich region {494-628}
MOTIF: N-glycosylation site {N557}
N-glycosylation site {N602}
EGF domain {625-657}
MOTIF: cysteine residue {C629}
MODIFICATION: cysteine residue {C639}
cysteine residue {C633}
MODIFICATION: cysteine residue {C645}
cysteine residue {C639}
MODIFICATION: cysteine residue {C629}
cysteine residue {C645}
MODIFICATION: cysteine residue {C633}
cysteine residue {C647}
MODIFICATION: cysteine residue {C656}
cysteine residue {C656}
MODIFICATION: cysteine residue {C647}
transmembrane domain {666-686}
Database Correlations
OMIM 606188
UniProt Q9UKQ2
PFAM correlations
Entrez Gene 10863
Kegg hsa:10863
References
UniProt :accession Q9UKQ2