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A disintegrin & metalloproteinase domain 21 (ADAM21)
Function:
- may be involved in sperm maturation &/or fertilization
- may also be involved in epithelia functions
- associated with establishing & maintaining gradients of ions or nutrients
- no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated
Cofactor: binds 1 Zn+2 per subunit (putative)
Structure:
- a tripeptide motif (VGE) within disintegrin-like domain could be involved in binding to egg integrin receptor & thus could mediate sperm/egg binding
- cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion
- conserved Cys present in the cysteine-switch motif binds the catalytic Zn+2, thus inhibiting the enzyme
- dissociation of Cys from Zn+2 upon activation- peptide release activates the enzyme
- contains 1 disintegrin domain
- contains 1 EGF-like domain
- contains 1 peptidase M12B domain
Compartment: membrane
Notes:
- may be the functional equivalent of fertilin alpha (ADAM1) which is a pseudogene in human
General
ADAM (A disintegrin & metalloproteinase domain); MDC (metalloproteinase, disintegrin, cysteine-rich) protein
glycoprotein
Properties
SIZE: MW = 81 kD
entity length = 722 aa
MOTIF: N-glycosylation site {N164}
metalloprotease domain {197-398}
MOTIF: N-glycosylation site {N227}
cysteine residue {C316}
MODIFICATION: cysteine residue {C393}
active site
SITE: E342
cysteine residue {C356}
MODIFICATION: cysteine residue {C378}
N-glycosylation site {N377}
cysteine residue {C378}
MODIFICATION: cysteine residue {C356}
cysteine residue {C393}
MODIFICATION: cysteine residue {C316}
disintegrin domain {406-492}
MOTIF: N-glycosylation site {N437}
cysteine residue {C464}
MODIFICATION: cysteine residue {C477}
cysteine residue {C477}
MODIFICATION: cysteine residue {C464}
N-glycosylation site {N478}
cysteine-rich region {493-633}
MOTIF: N-glycosylation site {N546}
N-glycosylation site {N600}
EGF domain {634-663}
MOTIF: cysteine residue {C634}
MODIFICATION: cysteine residue {C645}
cysteine residue {C639}
MODIFICATION: cysteine residue {C651}
cysteine residue {C645}
MODIFICATION: cysteine residue {C634}
cysteine residue {C651}
MODIFICATION: cysteine residue {C639}
cysteine residue {C653}
MODIFICATION: cysteine residue {C662}
cysteine residue {C662}
MODIFICATION: cysteine residue {C653}
transmembrane domain {682-702}
Database Correlations
OMIM 603713
UniProt Q9UKJ8
PFAM correlations
Entrez Gene 8747
Kegg hsa:8747
References
UniProt :accession Q9UKJ8