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A disintegrin & metalloproteinase domain 20 (ADAM20)
Function:
- may be involved in sperm maturation &/or fertilization
- no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated
Cofactor: binds 1 Zn+2 per subunit (putative)
Structure:
- a tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor & thus could mediate sperm/egg binding
- cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion
- conserved Cys present in the cysteine-switch motif binds the catalytic Zn+2, thus inhibiting the enzyme
- dissociation of Cys from Zn+2 upon the activation- peptide release activates the enzyme
- contains 1 disintegrin domain
- contains 1 EGF-like domain
- contains 1 peptidase M12B domain
Compartment: membrane
Expression: testis specific
Notes:
- may be the functional equivalent of ADAM1 (fertilin alpha) which is a pseudogene in human
General
ADAM (A disintegrin & metalloproteinase domain); MDC (metalloproteinase, disintegrin, cysteine-rich) protein
glycoprotein
testis-specific protein
Properties
SIZE: MW = 82 kD
entity length = 726 aa
COMPARTMENT: cellular compartment
CELL: unspecified cell type
WITHIN: testis
MOTIF: N-glycosylation site {N191}
metalloprotease domain {207-384}
MOTIF: N-glycosylation site {N226}
cysteine residue {C317}
MODIFICATION: cysteine residue {C394}
active site
SITE: E343
cysteine residue {C357}
MODIFICATION: cysteine residue {C379}
N-glycosylation site {N378}
cysteine residue {C379}
MODIFICATION: cysteine residue {C357}
cysteine residue {C394}
MODIFICATION: cysteine residue {C317}
disintegrin domain {407-493}
MOTIF: N-glycosylation site {N438}
cysteine residue {C465}
MODIFICATION: cysteine residue {C478}
cysteine residue {C478}
MODIFICATION: cysteine residue {C465}
N-glycosylation site {N479}
cysteine-rich region {494-634}
MOTIF: N-glycosylation site {N587}
EGF domain {635-663}
MOTIF: cysteine residue {C635}
MODIFICATION: cysteine residue {C646}
cysteine residue {C640}
MODIFICATION: cysteine residue {C652}
cysteine residue {C646}
MODIFICATION: cysteine residue {C635}
cysteine residue {C652}
MODIFICATION: cysteine residue {C640}
cysteine residue {C654}
MODIFICATION: cysteine residue {C663}
cysteine residue {C663}
MODIFICATION: cysteine residue {C654}
transmembrane domain {694-714}
Database Correlations
OMIM 603712
UniProt O43506
PFAM correlations
Entrez Gene 8748
Kegg hsa:8748
References
UniProt :accession O43506