azurocidin; cationic antimicrobial protein CAP37; heparin-binding protein; HBP (AZU1)

Function: - neutrophil granule-derived antibacterial & monocyte- & fibroblast-specific chemotactic glycoprotein - binds heparin - the cytotoxic action is limited to many species of Gram-negative bacteria - specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope - may play a role in mediating recruitment of monocytes in the second wave of inflammation - antibacterial activity against P aeruginosa - activity is inhibited by LPS from P aeruginosa acting alone - does not have antimicrobial activity against - A actinomycetemcomitans ATCC 29532,, NCTC 9709 or FDC-Y4, - H aphrophilus ATCC 13252 - E corrodens ATCC 23834 - C sputigena ATCC 33123 - Capnocytophaga sp ATCC 33124, ATCC 27872 - E.coli ML 35 - antibacterial activity against C sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G Structure: - monomer - belongs to the peptidase S1 family, elastase subfamily - contains 1 peptidase S1 domain Compartment: - cytoplasmic granules of neutrophils

General

glycoprotein secreted protein

Properties

SIZE: entity length = 251 aa MW = 27 kD COMPARTMENT: cytoplasm MOTIF: signal sequence {1-26} S1 domain {27-244} MOTIF: Possesses antibiotic activity {46-70} Hydrophobic {52-84} cysteine residue {C52} MODIFICATION: cysteine residue {C68} cysteine residue {C68} MODIFICATION: cysteine residue {C52} N-glycosylation site {N126} N-glycosylation site {N140} cysteine residue {C149} MODIFICATION: cysteine residue {C207} N-glycosylation site {N171} cysteine residue {C180} MODIFICATION: cysteine residue {C186} cysteine residue {C186} MODIFICATION: cysteine residue {C180} cysteine residue {C197} MODIFICATION: cysteine residue {C222} cysteine residue {C207} MODIFICATION: cysteine residue {C149} cysteine residue {C222} MODIFICATION: cysteine residue {C197}

Database Correlations

OMIM 162815 UniProt P20160 Pfam PF00089 Entrez Gene 566 Kegg hsa:566

References

UniProt :accession P20160