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ADP-ribosylation factor GTPase-activating protein 1; ARF GTPase activating protein 1; ARF GAP 1; ADP-ribosylation factor 1 GTPase-activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein (ARFGAP1, ARF1GAP, ASAP1, DDEF1, KIAA1249)
Function:
- GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1)
- role in membrane trafficking & /or vesicle transport
- promotes hydrolysis of the ARF1-bound GTP & is required for dissociation of coat proteins from Golgi-derived membranes & vesicles, a prerequisite for vesicle's fusion with the target compartment
- probably regulates ARF1-mediated transport via interaction with the KDELR proteins & TMED2
- overexpression induces redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated
- activity is stimulated by phosphoinosides
- interacts with ARF1
- interacts with the COPI coat proteins, KDELR1 & TMED2
- interaction with TMED2 inhibits the GAP activity (putative)
Inhibition:
- inhibited by phosphatidylcholine (putative)
Structure:
- the region downstream of Arf-GAP domain is essential to GAP activity in vivo
- this region may be required for its targeting to Golgi membranes (putative)
- contains 1 Arf-GAP domain
Compartment:
- cytoplasm (putative)
- Golgi apparatus (putative)
- associates with the Golgi complex
Alternative splicing: named isoforms=3
Related
Arf-GAP with SH3 domain, ANK repeat & PH domain-containing protein 1; 130 kD phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ADP-ribosylation factor-directed GTPase-activating protein 1; ARF GTPase-activating protein 1; development & differentiation-enhancing factor 1; DEF-1; differentiation-enhancing factor 1; PIP2-dependent ARF1 GAP (ASAP1, DDEF1, KIAA1249)
General
ankyrin repeat domain protein (ANKRD)
ADP-ribosylation factor GTPase activating protein (ARF-GAP)
zinc finger protein
Properties
SIZE: entity length = 406 aa
MW = 45 kD
COMPARTMENT: cytoplasm
golgi
MOTIF: Arf-GAP domain
NAME: Arf-GAP domain
SITE: 7-124
MOTIF: Zn finger C4-type
SITE: 22-45
EFFECTOR-BOUND: Zn+2
Thr phosphorylation site {T135}
Thr phosphorylation site {T189}
Ser phosphorylation site {S246}
Ser phosphorylation site {S304}
Ser phosphorylation site {S343}
Ser phosphorylation site {S345}
Ser phosphorylation site {S348}
Ser phosphorylation site {S361}
Database Correlations
OMIM 608377
UniProt Q8N6T3
Pfam PF01412
Entrez Gene 55738
Kegg hsa:55738
References
UniProt :accession Q8N6T3