Arf-GAP with SH3 domain, ANK repeat & PH domain-containing protein 1; 130 kD phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ADP-ribosylation factor-directed GTPase-activating protein 1; ARF GTPase-activating protein 1; development & differentiation-enhancing factor 1; DEF-1; differentiation-enhancing factor 1; PIP2-dependent ARF1 GAP (ASAP1, DDEF1, KIAA1249)

Function: - possesses phosphatidylinositol-4,5-bisphosphate dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) & ARF5 & lesser activity towards ARF6 - may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC & PIP2 - may function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes & possibly other cell types (putative) - role in ciliogenesis activity stimulated by phosphatidylinositol-4,5-bisphosphate (PIP2) (putative) - phosphorylated on Tyr by SRC (putative) - homodimer - interacts with SRC & CRK - interacts with RAB11FIP3 - interacts with PTK2B/PYK2 (putative) - interacts with CTTN - interacts (via SH3 domain) with APC Structure: - the PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors - contains 2 ANK repeats - contains 1 Arf-GAP domain - contains 1 PH domain - contains 1 SH3 domain Compartment: - cytoplasm (putative), membrane - predominantly cytoplasmic (putative) - partially membrane-associated (putative) Alternative splicing: named isoforms=2

General

ankyrin repeat domain protein (ANKRD) phosphoprotein zinc finger protein

Properties

SIZE: entity length = 1129 aa MW = 125 kD COMPARTMENT: cytoplasm MOTIF: PH domain {324-416} Arf-GAP domain NAME: Arf-GAP domain SITE: 439-560 MOTIF: Zn finger C4-type SITE: 454-477 EFFECTOR-BOUND: Zn+2 ankyrin repeat NAME: ankyrin repeat SITE: 600-632 ankyrin repeat NAME: ankyrin repeat SITE: 636-665 Ser phosphorylation site {S717} proline-rich region SITE: 783-993 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S839} Ser phosphorylation site {S843} Ser phosphorylation site {S1008} Ser phosphorylation site {S1027} src homology 3 [SH3] domain SITE: 1067-1129

Database Correlations

OMIM 605953 UniProt Q9ULH1 PFAM correlations Entrez Gene 50807 Kegg hsa:50807

References

UniProt :accession Q9ULH1