Contents

Search

apoptosis-inducing factor 1, mitochondrial; programmed cell death protein 8 (AIFM1, AIF, PDCD8)

Function: - probable oxidoreductase that acts as a caspase- independent mitochondrial effector of apoptotic cell death - AIF is normally in mitochondria, translocates to the nucleus during apoptosis & induces mitochondria to release cytochrome C & caspase 9 - Bcl-2 inhibits mitochondrial release of AIF but otherwise does not affect apoptotis activity - recombinant AIF causes chromatin condensation & DNA fragmentation in isolated nuclei - binds to DNA in (sequence-independent) - effects of AIF are independent of caspases [3] - AIF is essential for programmed cell death during early morphogenesis, ie. cavitation of embryoid bodies - interacts with XIAP Cofactor: FAD Structure: - belongs to the FAD-dependent oxidoreductase family Compartment: - mitochondria intermembrane space - nucleus - translocated to the nucleus upon induction of apoptosis Alternative splicing: - named isoforms=3

Related

apoptosis programmed cell death protein

General

flavoprotein mitochondrial protein oxidoreductase phosphoprotein pro apoptotic protein

Properties

SIZE: entity length = 613 aa MW = 67 kD COMPARTMENT: mitochondria cell nucleus MOTIF: Thr phosphorylation site {T105} FAD-dependent oxidoreductase {134-483} MOTIF: cofactor-binding site [138-142] COFACTOR-BOUND: flavin adenine dinucleotide cofactor-binding site [164-165] COFACTOR-BOUND: flavin adenine dinucleotide binding site SITE: 172-172 FOR-BINDING-OF: flavin adenine dinucleotide binding site SITE: 177-177 FOR-BINDING-OF: flavin adenine dinucleotide binding site SITE: 233-233 FOR-BINDING-OF: flavin adenine dinucleotide Ser phosphorylation site {S268} cofactor-binding site [285-285] FOR-BINDING-OF: flavin adenine dinucleotide cofactor-binding site [438-438] FOR-BINDING-OF: flavin adenine dinucleotide nuclear translocation signal {446-451} cofactor-binding site [454-455] COFACTOR-BOUND: flavin adenine dinucleotide binding site SITE: 483-483 FOR-BINDING-OF: flavin adenine dinucleotide

Database Correlations

OMIM 300169 UniProt O95831 PFAM correlations LOCUS-LINK correlations Kegg hsa:51060

References

  1. UniProt :accession O95831
  2. Atlas of Genetics & Cytogenetics in Oncology & Haematology http://atlasgeneticsoncology.org/genes/AIFM1ID44053chXq25.html
  3. Susin SA et al Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397:441-7 1999 PMID: 9989411
  4. Joza N et al Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. Nature 410:549-54, 2001 PMID: 11279485
  5. Daugas E et al Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J 14:729, 2000 PMID: 10744629