Search
anoctamin-1; transmembrane protein 16A; discovered on gastrointestinal stromal tumors protein 1; oral cancer overexpressed protein 2; tumor amplified & overexpressed sequence 2 (TMEM16A, DOG1, ORAOV2, TAOS2, ANO1)
Function:
- Ca+2-activated chloride channel
- role in transepithelial anion transport & smooth muscle contraction
- required for the normal functioning of the interstitial cells of Cajal which generate electrical pacemaker activity in gastrointestinal smooth muscles
- major contributor to basal & stimulated chloride conductance in airway epithelial cells
- role in tracheal cartilage development
- ATP & calmodulin essential for activation
- channel activity is inhibited by CFTR protein & by chloride inhibitors such as niflumic acid (NFA) & 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS)
- interacts with CFTR
- interacts with TRPV4 (putative)
Structure:
- homodimer
- the region spanning the 5th & 6th transmembrane domains probably forms the pore-forming region
- belongs to the anoctamin family,
Compartment:
- cell membrane
- cytoplasmic localization seen in neoplastic cells of head & neck squamous cell carcinoma tumors
Alternative splicing: named isoforms=3
Expression:
- broadly expressed with higher levels in liver, skeletal muscle & gastrointestinal muscles
Notes:
- the term 'anoctamin' was coined because these channels are anion selective & have 8 (OCT) transmembrane segments*
* not certain that all members of this family are anion channels or have the 8-transmembrane topology
Related
DOG1 (anoctamin-1) Ag in tissue
General
Cl- channel
glycoprotein
transmembrane 8 protein
Properties
SIZE: MW = 114 kD
entity length = 986 aa
COMPARTMENT: cytoplasm
MOTIF: cytoplasmic domain {1-333}
MOTIF: Ser phosphorylation site {S107}
Ser phosphorylation site {S196}
transmembrane domain {334-354}
exoplasmic loop {355-402}
transmembrane domain {403-423}
cytoplasmic loop {424-519}
transmembrane domain {520-540}
exoplasmic loop {541-565}
transmembrane domain {566-586}
cytoplasmic loop {587-606}
transmembrane domain {607-627}
exoplasmic loop {628-731}
transmembrane domain {732-752}
cytoplasmic loop {753-791}
transmembrane domain {792-812}
exoplasmic loop {813-882}
MOTIF: N-glycosylation site {N832}
transmembrane domain {883-903}
cytoplasmic domain {904-986}
ION-PERMEABILITY: Cl-
Database Correlations
OMIM 610108
UniProt Q5XXA6
PFAM correlations
Entrez Gene 55107
Kegg hsa:55107
References
- UniProt :accession Q5XXA6
- UniProt PubMed refs
- PMID: 21984732
- PMID: 984732
- PMID: 22946059
- PMID: 22178883
- PMID: 20056604
- PMID: 056604
- PMID: 178883