Search
ankyrin-1; erythrocyte ankyrin; ankyrin-R (ANK1 ANK)
Function:
- attaches integral membrane proteins to cytoskeletal elements
- binds
a) erythrocyte membrane protein band 4.2
b) Na-K ATPase
c) lymphocyte membrane protein GP85
d) cytoskeletal proteins fodrin, tubulin, vimentin & desmin
- erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein
- in skeletal muscle, isoform Mu17 together with obscurin may provide amolecular link between the sarcoplasmic reticulum & myofibrils
- regulated by phosphorylation
- interacts with a number of integral membrane proteins & cytoskeletal proteins
- binds SPTB/spectrin (beta chain) through a 70 AA N-terminal region of the 62 kD domain, & the C-terminal of SLC4A1/erythrocyte membrane protein band 3 through the ankyrin repeat region
- also interacts with TTN/titin
- isoform Mu17 interacts with OBSCN isoform 3/obscurin
Structure:
- palmitoylated
- the 55 kD regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) & SLC4A1/erythrocyte membrane protein band 3
- the ANK repeat region forms a spiral around a large central cavity & is involved in binding of ion transporters
- contains 23 ANK repeats
- contains 1 death domain
- contains 1 ZU5 domain
Compartment:
- isoform Er1: cytoplasm, cytoskeleton; other erythrocyte isoforms are probably located near the surface of erythrocytic plasma membrane
- isoform Mu17: membrane, cytoplasm, myofibril, sarcomere, M-band; colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells
- isoform Mu18, Mu19, Mu20: sarcoplasmic reticulum (probable)
Alternative splicing: named isoforms=21
Alternative promoter usage:
- major erythrocyte-specific isoform produced by alternative promoter usage
Expression:
- isoform Mu17, isoform Mu18, isoform Mu19 & isoform Mu20 are expressed in skeletal muscle
- isoform Br21 is expressed in brain
Pathology:
- defects in ANK1 are a cause of hereditary spherocytosis type 1
General
ankyrin
Properties
SIZE: entity length = 1881 aa
MW = 206 kD
COMPARTMENT: cytoplasm
MOTIF: 89 kDa {2-827}
MOTIF: ankyrin repeat
NAME: ankyrin repeat
SITE: 44-73
ankyrin repeat
NAME: ankyrin repeat
SITE: 77-106
ankyrin repeat
NAME: ankyrin repeat
SITE: 110-139
ankyrin repeat
NAME: ankyrin repeat
SITE: 143-172
ankyrin repeat
NAME: ankyrin repeat
SITE: 174-201
ankyrin repeat
NAME: ankyrin repeat
SITE: 205-234
ankyrin repeat
NAME: ankyrin repeat
SITE: 238-267
ankyrin repeat
NAME: ankyrin repeat
SITE: 271-300
ankyrin repeat
NAME: ankyrin repeat
SITE: 304-333
ankyrin repeat
NAME: ankyrin repeat
SITE: 337-366
ankyrin repeat
NAME: ankyrin repeat
SITE: 370-399
ankyrin repeat
NAME: ankyrin repeat
SITE: 403-432
ankyrin repeat
NAME: ankyrin repeat
SITE: 436-465
ankyrin repeat
NAME: ankyrin repeat
SITE: 469-498
ankyrin repeat
NAME: ankyrin repeat
SITE: 502-531
ankyrin repeat
NAME: ankyrin repeat
SITE: 535-564
ankyrin repeat
NAME: ankyrin repeat
SITE: 568-597
ankyrin repeat
NAME: ankyrin repeat
SITE: 601-630
ankyrin repeat
NAME: ankyrin repeat
SITE: 634-663
ankyrin repeat
NAME: ankyrin repeat
SITE: 667-696
ankyrin repeat
NAME: ankyrin repeat
SITE: 700-729
ankyrin repeat
NAME: ankyrin repeat
SITE: 733-762
ankyrin repeat
NAME: ankyrin repeat
SITE: 766-795
Ser phosphorylation site {S834}
Ser phosphorylation site {S856}
ZU5 {911-1018}
Tyr phosphorylation site {Y1073}
55 kDa regulatory {1383-1881}
MOTIF: death domain
NAME: death domain
SITE: 1403-1487
Thr phosphorylation site {T1684}
Ser phosphorylation site {S1686}
Database Correlations
OMIM correlations
UniProt P16157
PFAM correlations
Entrez Gene 286
Kegg hsa:286
References
- UniProt :accession P16157
- Wikipedia; Note: ankyrin entry
http://en.wikipedia.org/wiki/ankyrin
- Entrez Gene :accession 286