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alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 (alkylated DNA repair protein alkB homolog 2, oxy DC1, ALKBH2, ABH2)
Function:
- dioxygenase
- repairs alkylated DNA & RNA containing 1-methyladenine & 3-methylcytosine by oxidative demethylation
- strong preference for double-stranded DNA
- low efficiency with single-stranded substrates
- requires molecular oxygen, alpha-ketoglutarate & iron
- activated by ascorbate
Cofactor: binds 1 Fe+2 per subunit
Structure: belongs to the alkB family
Compartment:
- nucleus
- detected in replication foci during S-phase
Expression:
- detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver & urinary bladder
General
alkylated DNA repair protein alkB homolog (ALKBH)
dioxygenase
metalloprotein
nuclear protein
Properties
SIZE: MW = 29 kD
entity length = 261 aa
COMPARTMENT: cell nucleus
MOTIF: binding site
SITE: 91-91
FOR-BINDING-OF: Substrate
binding site
SITE: 110-110
FOR-BINDING-OF: Substrate
Substrate binding {122-124}
binding site
SITE: 159-159
FOR-BINDING-OF: Alpha-ketoglutarate
binding site
SITE: 161-161
FOR-BINDING-OF: Alpha-ketoglutarate
Iron [Fe]-binding site
SITE: 171-171
Iron [Fe]-binding site
SITE: 173-173
binding site
SITE: 174-174
FOR-BINDING-OF: Substrate
Iron [Fe]-binding site
SITE: 236-236
Alpha-ketoglutarate binding {248-254}
Database Correlations
UniProt Q6NS38
Pfam PF03171
References
UniProt :accession Q6NS38