alkylated DNA repair protein alkB homolog 1; alpha-ketoglutarate-dependent dioxygenase ABH1; DNA lyase ABH1 (ALKBH1, ABH, ABH1, ALKBH)

Function: - dioxygenase - repairs alkylated single-stranded DNA & RNA containing 3-methylcytosine by oxidative demethylation - requires molecular oxygen, alpha-ketoglutarate & iron - may have a role in placental trophoblast lineage differentiation - as DNA lyase activity & introduces double-stranded breaks at abasic sites - cleaves both single-stranded DNA & double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites - DNA lyase activity does not require alpha-ketoglutarate & iron - the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar & a product with a terminal 5'-phosphate - interacts with DNAJB6 (putative) Cofactor: - binds 1 Fe⁺² ion per subunit Structure: - monomer - belongs to the alkB family - contains 1 Fe2OG dioxygenase domain Compartment: - mitochondria, nucleus (putative) - mainly localizes in euchromatin, largely excluded from heterochromatin & nucleoli (putative) Expression: ubiquitous

General

alkylated DNA repair protein alkB homolog (ALKBH) dioxygenase mitochondrial protein nuclear protein

Properties

SIZE: entity length = 389 aa MW = 44 kD COMPARTMENT: mitochondria cell nucleus MOTIF: binding site SITE: 170-170 FOR-BINDING-OF: Substrate Substrate binding {177-179} Fe2OG dioxygenase {208-347} MOTIF: Alpha-ketoglutarate binding {220-222} Iron [Fe]-binding site SITE: 231-231 Iron [Fe]-binding site SITE: 233-233 binding site SITE: 261-261 FOR-BINDING-OF: Substrate Iron [Fe]-binding site SITE: 287-287 Alpha-ketoglutarate binding {338-344}

Database Correlations

OMIM 605345 UniProt Q13686 Pfam PF13532 Entrez Gene 8846 Kegg hsa:8846 ENZYME 4.2.99.18

References

UniProt :accession Q13686