mediator of RNA polymerase II transcription subunit 15; activator-recruited cofactor 105 kD component; ARC105; CTG repeat protein 7a; mediator complex subunit 15; positive cofactor 2 glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; TPA-inducible gene 1 protein; TIG-1; trinucleotide repeat-containing gene 7 protein (MED15, ARC105, CTG7A, PCQAP, TIG1, TNRC7)

Function: - component of the mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase 2- dependent genes - positively regulates the nodal signaling pathway - ubiquitinated by TRIM11, leading to proteasomal degradation - interacts with WWTR1 - interacts with TRIM11 Structure: - belongs to the mediator complex subunit 15 family Compartment: cytoplasm. nucleus Alternative splicing: named isoforms=3 Expression: - expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte & placenta - highest expression in placenta & peripheral blood - lowest expression in pancreas & kidney - induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) Polymorphism: - the poly-Gln region from amino acids 235-262 of MED15 is polymorphic - there are from 15-18 repeats in the Italian population

General

phosphoprotein transcription factor (TF)

Properties

SIZE: entity length = 788 aa MW = 87 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl SREBF1 interaction {9-73} glutamine-rich region {161-174} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {178-193} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {205-218} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {226-239} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {243-262} MOTIF: glutamine residue (SEVERAL) proline-rich region SITE: 266-515 MOTIF: proline residue (SEVERAL) glutamine-rich region {360-367} MOTIF: glutamine residue (SEVERAL) proline-rich region SITE: 449-456 MOTIF: proline residue (SEVERAL) nuclear translocation signal {547-564} proline-rich region SITE: 602-611 MOTIF: proline residue (SEVERAL) Thr phosphorylation site {T603}

Database Correlations

OMIM 607372 UniProt Q96RN5 Pfam PF09606 Entrez Gene 51586 Kegg hsa:51586

References

UniProt :accession Q96RN5