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activated RNA polymerase 2 transcriptional coactivator p15; positive cofactor 4; activated RNA polymerase 2 transcription cofactor 4 (SUB1, PC4, RPO2TC1, p14)
Function:
- general coactivator
- functions cooperatively with TAFs & mediates functional interactions between upstream activators & the general transcriptional machinery
- may be involved in stabilizing the multiprotein transcription complex
- binds single-stranded DNA
- also binds, in vitro, non-specifically to double-stranded DNA (ds DNA)
- activity is controlled by protein kinases that target the regulatory region
- phosphorylation inactivates both ds DNA-binding & cofactor function, but does not affect binding to ssDNA
- seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region
- interacts with CSTF2
Structure:
- homodimer
- belongs to the transcriptional coactivator PC4 family
- mass spec: mass=14266; mass_error=4; method=MALDI [2] mass spec: mass=14263; method=electrospray [3]
Compartment: nucleus
General
nuclear protein
phosphoprotein
Properties
SIZE: entity length = 127 aa
MW = 14 kD
COMPARTMENT: cell nucleus
MOTIF: Regulatory {2-50}
MOTIF: serine-rich region {4-19}
MOTIF: serine residue (SEVERAL)
Ser phosphorylation site {S9}
Ser phosphorylation site {S10}
Ser phosphorylation site {S11}
Ser phosphorylation site {S12}
Ser phosphorylation site {S13}
Ser phosphorylation site {S15}
Ser phosphorylation site {S17}
Ser phosphorylation site {S19}
lysine-rich region {23-53}
MOTIF: lysine residue (SEVERAL)
serine-rich region {50-58}
MOTIF: serine residue (SEVERAL)
peptide motif {50-51}
Ser phosphorylation site {S50}
Ser phosphorylation site {S52}
Ser phosphorylation site {S56}
Ser phosphorylation site {S58}
ssDNA interaction {77-101}
Database Correlations
OMIM 600503
UniProt P53999
Pfam PF02229
Entrez Gene 10923
Kegg hsa:1092
References
- UniProt :accession P53999
- PMID: 7809103
- PMID: 16689930