Search
17-ketogenic steroids in 24 hour urine
Indications:
- used in conjunction with metyrapone stimulation test
Reference interval:
-> adult
a) male: 5-23 mg/day (17-80 umol/day)
b) female: 3-15 mg/day (10-52 umol/day)
Clinical significance:
the test measures:
1) corticosteroids
2) their precursors
a) 17-hydroxyprogesterone
b) pregnane triol
2) their metabolites
a) cortols
b) cortolones
Increases:
1) pharmaceutical agents
a) in-vivo effects
-> ACTH, cortisone acetate, hydrocortisone
b) chemical interferences
-> cephalothin, digitoxin, meprobamate, phenothiazines, naproxen (m-dinitrobenzene reagent), penicillin, spironolactone
2) clinical disorders
a) Cushing's syndrome
b) congenital adrenal hyperplasia
c) obesity
d) acute illness
e) pregnancy
Decreases:
1) pharmaceutical agents
a) in-vivo effects
-> ampicillin, dexamethasone, estrogens, phenytoin, oral contraceptives, prednisone, prednisolone
b) chemical interferences
-> chlordiazepoxide, glucose, meglumine
2) clinical disorders
a) Addison's disease
b) hypopituitarism
c) hypothyroidism
d) generalized wasting syndrome
Method:
- colorimetric (Zimmerman)
Specimen:
1) urine, 24 hours
2) preserve with boric acid & refrigerate
3) stable for at least 2 weeks & for longer periods if frozen at -20 degrees C
Notes:
- largely replaced by specific assays of plasma & urine steroids
Related
17-hydroxycorticosteroids in 24 hour urine
17-ketosteroids in 24 hour urine
metyrapone stimulation test
General
24 hour urine
17-ketogenic steroids in urine
References
Clinical Guide to Laboratory Tests, 3rd ed. Teitz ed.,
W.B. Saunders, 1995