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urea in body fluid
Principle:
The Kodak Ektachem Clinical Chemistry Slide (BUN/UREA) is a dry, multilayered analytical element coated on a clear polyester support.
A 10 uL drop of patient sample is deposited on the slide & is evenly distributed by the spreading layer. Water & non- proteinaceous components then travel to the underlying reagent layer where the urease reaction generates ammonia. The ammonia then diffuses through the semipermeable membrane to react with an ammonia indicator.
The semipermeable membrane allows only ammonia to pass through to the color-forming layer where it reacts with the indicator.
After a fixed incubation period, the reflection density of the dye is measured spectrophotometrically through the polyester support using the white background of the spreading layer as a diffuse reflector.
Urease
H2NCONH2 + H2O -----------> 2NH3 + CO2
NH3 + Ammonia Indicator ----------> Dye
Specimen:
- specimens are collected in a red top vacutainer & should be separated immediately from the cells after collection
- minimum sample size 0.5 milliliter: with an optimum size of 1.0 mL or larger
Related
urea (carbamide)
Specific
urea in amniotic fluid
urea in dialysis fluid
urea in gastric fluid
urea in peritoneal fluid
urea in pleural fluid
urea in serum/plasma//blood
urea in vitreous fluid
General
general chemistry test
References
- Urea Nitrogen, Body Fluid
Laboratory Test Directory ARUP: 20183