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ubiquitin-like PHD & RING finger domain-containing protein 1; E3 ubiquitin-protein ligase UHRF1; inverted CCAAT box-binding protein of 90 kDa; nuclear protein 95; nuclear zinc finger protein Np95; HuNp95; RING finger protein 106; transcription factor ICBP90; ubiquitin-like-containing PHD & RING finger domains protein 1 (UHRF1, ICBP90, NP95, RNF106)

Function: 1) E3 ubiquitin ligase protein, - ubiquitin conjugation, 3rd step 2) methylation-dependent transcriptional regulation - binds methylated CpG containing oligonucleotides 3) binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, activates TOP2A expression 4) G1/S transition 5) DNA repair 6) chromosomal stability 7) interactions: histones H3, H1, H2B, HDAC1, UHRF1BP1 8) phosphorylation on Ser enhances DNA-binding activity 9) ubiquitination leads to proteasomal degradation 10) polyubiquitination stimulated by DNA damage Structure: - the RING finger is required for ubiquitin ligase activity - the YDG domain mediates the interaction with histone H3 - contains 1 PHD-type Zn+2 finger - contains 2 RING-type Zn+2 fingers - contains 1 ubiquitin-like domain - contains 1 YDG domain Compartment: nucleus Expression: - expressed in: thymus, bone marrow, testis, lung, heart, fetal thymus, liver, kidney - up-regulated in proliferating cells (induced by E2F1), down-regulated in quiescent cells Pathology: - overexpressed in breast cancer - down-regulated upon adriamycin-induced DNA damage, TP53/p53 & CDKN1A-dependent

General

phosphoprotein ring finger protein E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein

Properties

SIZE: entity length = 793 aa MW = 90 kD COMPARTMENT: cell nucleus MOTIF: Ubiquitin-like {1-78} MOTIF: Ser phosphorylation site {S76} Ser phosphorylation site {S91} Ser phosphorylation site {S287} Ser phosphorylation site {S298} Zn finger PHD-type NAME: Zn finger PHD-type SITE: 310-366 EFFECTOR-BOUND: Zn+2 Methyl-CpG binding and interaction with HDAC1 {386-575} YDG {419-582} Ser phosphorylation site {S639} Ser phosphorylation site {S707} RING-finger {724-763} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif

Database Correlations

OMIM 607990 UniProt Q96T88 PFAM correlations Entrez Gene 29128 Kegg hsa:29128

References

  1. UniProt :accession Q96T88
  2. NIEHS-SNPs http://egp.gs.washington.edu/data/uhrf1/