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tRNA-splicing endonuclease complex
Function:
1) identification & cleavage of splice sites in pre-tRNA.
2) cleaves pre-tRNA at the 5' & 3' splice sites to release intron
- products are an intron & 2 tRNA half-molecules bearing 2',3' cyclic phosphate & 5'-OH termini
3) no conserved sequences at the splice sites, but intron is invariably located at the same site in the gene, placing splice sites an invariant distance from the constant structural features of the tRNA
4) mRNA processing via association with pre-mRNA 3' end processing factors
5) links pre-tRNA splicing & pre-mRNA 3' end formation
Structure:
- heterotetramer consisting of: SEN2, SEN15, SEN34, SEN54
- associated with proteins of the pre-mRNA 3' end processing complex
- CLP1, CPSF1, CPSF4, CSTF2
General
endoribonuclease
molecular complex
Properties
COMPARTMENT: cell nucleus
MOTIF: active site
SUBUNITS: tRNA-splicing endonuclease subunit sen2
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S240}
tyrosine residue {Y369}
histidine residue {H377}
Ser phosphorylation site {S408}
Ser phosphorylation site {S411}
Ser phosphorylation site {S415}
lysine residue {K416}
tRNA-splicing endonuclease subunit Sen15
COMPARTMENT: cell nucleus
tRNA-splicing endonuclease subunit sen34
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S134}
Ser phosphorylation site {S135}
Ser phosphorylation site {S136}
tyrosine residue {Y247}
histidine residue {H255}
lysine residue {K286}
tRNA-splicing endonuclease subunit Sen54
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S230}
Ser phosphorylation site {S235}
pre-mRNA cleavage complex 2 protein Clp1
COMPARTMENT: cell nucleus
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 121-128
cleavage & polyadenylation specificity factor subunit 1
COMPARTMENT: cell nucleus
MOTIF: Thr phosphorylation site {T739}
nuclear translocation signal {893-908}
cleavage & polyadenylation specificity factor subunit 4
COMPARTMENT: cell nucleus
MOTIF: Zinc finger
NAME: Zinc finger
SITE: 35-61
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 62-89
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 90-117
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 118-142
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 143-169
EFFECTOR-BOUND: Zn+2
Ser phosphorylation site {S200}
Ser phosphorylation site {S202}
Ser phosphorylation site {S211}
Ser phosphorylation site {S212}
Zn finger C2HC-type
SITE: 243-260
EFFECTOR-BOUND: Zn+2
cleavage stimulation factor 64 kD subunit
COMPARTMENT: cell nucleus
MOTIF: RNP motif
NAME: RNP motif
SITE: 16-94
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
NAME: ribonucleoprotein-1 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
ribonucleoprotein-2 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
CSTF3/SYMPK interaction {108-248}
MOTIF: Ser phosphorylation site {S154}
proline-rich region
SITE: 198-409
MOTIF: proline residue (SEVERAL)
M-E-A-R-[AG] repeats {410-469}
MOTIF: consensus repeat {410-414}
consensus repeat {415-419}
consensus repeat {420-424}
consensus repeat {425-429}
consensus repeat {430-434}
consensus repeat {435-439}
consensus repeat {440-444}
consensus repeat {445-449}
consensus repeat {450-454}
consensus repeat {455-459}
consensus repeat {460-464}
consensus repeat {465-469}
proline-rich region
SITE: 470-526
MOTIF: proline residue (SEVERAL)
RPO2TC1 interaction {514-577}
MOTIF: Ser phosphorylation site {S518}
Ser phosphorylation site {S524}
References
UniProt :accession Q7Z6J9
Components
cleavage & polyadenylation specificity factor subunit 1; cleavage & polyadenylation specificity factor 160 kD subunit; CPSF 160 kD subunit (CPSF1, CPSF160)
cleavage & polyadenylation specificity factor subunit 4; cleavage & polyadenylation specificity factor 30 kD subunit; CPSF 30 kD subunit; NS1 effector domain-binding protein 1; Neb-1; No arches homolog (CPSF4 CPSF30 NAR NEB1)
cleavage stimulation factor 64 kD subunit; CSTF 64 kD subunit; CstF-64; CF-1 64 kD subunit (CSTF2)
pre-mRNA cleavage complex 2 protein Clp1 (CLP1, HEAB)
tRNA-splicing endonuclease subunit Sen15 (tRNA-intron endonuclease Sen15, HsSen15, TSEN15, SEN15)
tRNA-splicing endonuclease subunit sen2; tRNA-intron endonuclease sen2; Hssen2 (TSEN2 SEN2)
tRNA-splicing endonuclease subunit sen34; leukocyte receptor cluster member 5; tRNA-intron endonuclease sen34; Hssen34 (TSEN34, LENG5, SEN34)
tRNA-splicing endonuclease subunit Sen54 (tRNA-intron endonuclease Sen54, HsSen54, TSEN54, SEN54)