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E3 ubiquitin-protein ligase TRIM32; tripartite motif-containing protein 32; zinc finger protein HT2A; 72 kD Tat-interacting protein (TRIM32, HT2A)
Function:
- has an E3 ubiquitin ligase activity
- ubiquitinates DTNBP1 (dysbindin) & promotes its degradation
- protein modification; protein ubiquitination
- ubiquitinated
- interacts with DTNBP1
- self-associates
Structure:
- belongs to the TRIM/RBCC family
- contains 1 B box-type Zn+2 finger
- contains 5 NHL repeats
- contains 1 RING-type Zn+2 finger
Compartment:
- cytoplasm
- localized in cytoplasmic bodies, often located around the nucleus
Expression:
- spleen, thymus, prostate, testis, ovary, intestine, colon & skeletal muscle
Pathology:
- defects in TRIM32 are the cause of
a) limb-girdle muscular dystrophy type 2H
b) Bardet-Biedl syndrome type 11
- may play a role in mediating the biological activity of the HIV-1 Tat protein in vivo
a) binds specifically to the activation domain of HIV-1 Tat
b) can also interact with the HIV-2 & EIAV Tat proteins in vivo
General
phosphoprotein
tripartite motif-containing protein (trim protein)
E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein
Properties
SIZE: entity length = 653 aa
MW = 72 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: alanine-rich region {2-6}
MOTIF: alanine residue (SEVERAL)
acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
RING-finger {20-65}
EFFECTOR-BOUND: Zn+2
FOR-BINDING-OF: DNA motif
zinc finger B box
SITE: 103-133
EFFECTOR-BOUND: Zn+2
coiled coil {138-197}
Ser phosphorylation site {S328}
Ser phosphorylation site {S335}
Ser phosphorylation site {S339}
NHL repeat domain {358-401}
NHL repeat domain {415-458}
NHL repeat domain {459-499}
NHL repeat domain {562-605}
NHL repeat domain {606-646}
Database Correlations
OMIM correlations
MORBIDMAP 602290
UniProt Q13049
PFAM correlations
Entrez Gene 22954
Kegg hsa:22954
References
- UniProt :accession Q13049
- Leiden muscular Dystrophy pages; Note: TRIM32
http://www.dmd.nl/trim32_home.html
- GeneReviews
http://www.ncbi.nlm.nih.gov/sites/genetests/lab/gene/TRIM32
- OMIM :accession 602290