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transcription factor Sp1 (SP1, TSFP1)

Function: - recognizes & binds to Sp1 sites - one of the transcription factors that is directly influenced by reactive oxygen species & proinflammatory cytokines - binds to GC box promoters elements & selectively activates mRNA synthesis from genes that contain functional recognition sites - can interact with G/C-rich motifs from serotonin receptor promoter - interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF & PHC2 - interacts with varicella-zoster virus IE62 protein & HIV-1 Vpr Structure: - O-glycosylated; contains N-acetylglucosamine side chains - belongs to the Sp1 C2H2-type Zn+2-finger protein family - contains 3 C2H2-type Zn+2 fingers Compartment: - nucleus, cytoplasm - nuclear location is regulated by glycosylation & phosphorylation - insulin promotes nuclear location - glucagon promotes cytoplasmic location Expression: - induced by insulin - up-regulated in adenocarcinomas of the stomach (at protein level)

Interactions

molecular events

Related

SP1-binding site

General

C2H2 type zinc finger protein glycoprotein phosphoprotein redox-modulated transcription factor Sp1 transcription factor family

Properties

SIZE: entity length = 785 aa MW = 81 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: Repressor {1-82} MOTIF: Ser phosphorylation site {S7} threonine-rich region {36-143} MOTIF: threonine residue (SEVERAL) Ser phosphorylation site {S59} proteolytic site {63-64} Ser phosphorylation site {S101} transcriptional activation domain SITE: 146-251 transcriptional activation domain SITE: 261-495 MOTIF: threonine-rich region {271-379} MOTIF: threonine residue (SEVERAL) Thr phosphorylation site {T278} Thr phosphorylation site {T453} Ser glycosylation site {S491} transcriptional activation domain SITE: 496-610 Ser phosphorylation site {S612} Ser glycosylation site {S612} binding site SITE: 619-785 FOR-BINDING-OF: Varicella zoster MOTIF: Zn finger C2H2-type SITE: 626-650 EFFECTOR-BOUND: Zn+2 Thr phosphorylation site {T640} Thr glycosylation site {T640} Ser phosphorylation site {S641} Ser glycosylation site {S641} Thr phosphorylation site {T651} Zn finger C2H2-type SITE: 656-680 EFFECTOR-BOUND: Zn+2 Thr phosphorylation site {T668} Ser phosphorylation site {S670} Thr phosphorylation site {T681} Zn finger C2H2-type SITE: 686-708 EFFECTOR-BOUND: Zn+2 Ser glycosylation site {S698} Ser phosphorylation site {S702} Ser glycosylation site {S702} Domain D {708-785} Thr phosphorylation site {T739}

Database Correlations

OMIM 189906 UniProt P08047 Pfam PF00096 Entrez Gene 6667 Kegg hsa:6667

References

  1. Kadonaga et al Science 242:1566 1988
  2. Molecular Cell Biology (2nd ed) Darnell J; Lodish H & Baltimore D (eds), Scientific American Books, WH Freeman, NY 1990, pg 296
  3. Berk AJ. Regulation of eukaryotic transcription factors by post- translational modification. Biochim Biophys Acta. 1989 Nov 2;1009(2):103-9. Review. PMID: 2529903
  4. Ross CA, McInnis MG, Margolis RL, Li SH. Genes with triplet repeats: candidate mediators of neuropsychiatric disorders. Trends Neurosci. 1993 Jul;16(7):254-60. Review. PMID: 7689767
  5. Lavrovsky Y, Chatterjee B, Clark RA, Roy AK. Role of redox-regulated transcription factors in inflammation, aging and age-related diseases. Exp Gerontol. 2000 Aug;35(5):521-32. Review. PMID: 10978675
  6. UniProt :accession P08047

Component-of

CREST-BRG1 complex