tight junction protein ZO-1; zonula occludens protein 1; zona occludens protein 1; tight junction protein 1 (TJP1, ZO1)

Function: - N-terminal may be involved in transducing a signal required for tight junction assembly - C-terminal may have specific properties of tight junctions - alpha domain might be involved in stabilizing junctions - interacts with HSPA4 & KIRREL1 (putative) - homodimer, & heterodimer with TJP2/ZO-2 & TJP3/ZO-3 - interacts with occludin, claudins, CGN/cingulin, CXADR, GJA12, GJD3 & UBN1 Structure: - the 2nd PDZ domain mediates interaction with GJA12 - belongs to the MAGUK family - contains 1 guanylate kinase-like domain - contains 3 PDZ domain (DHR domain)s - contains 1 SH3 domain - contains 1 ZU5 domain Compartment: - cell membrane; peripheral membrane protein, cytoplasmic side - cell junction, tight junction - movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact Alternative splicing: named isoforms=2; long, short Expression: - the alpha-containing isoform is found in most epithelial cell junctions. - the short isoform is found both in endothelial cells & highly specialized epithelial junctions of renal glomeruli & Sertoli cells of the seminiferous tubules

General

phosphoprotein tight juntion protein or zona occludens protein

Properties

SIZE: entity length = 1748 aa MW = 195 kD COMPARTMENT: cytoplasm MOTIF: PDZ domain NAME: PDZ domain SITE: 23-110 Ser phosphorylation site {S125} Ser phosphorylation site {S131} Tyr phosphorylation site {Y132} Ser phosphorylation site {S166} Ser phosphorylation site {S168} Ser phosphorylation site {S175} Ser phosphorylation site {S178} Ser phosphorylation site {S179} PDZ domain NAME: PDZ domain SITE: 186-264 Ser phosphorylation site {S275} Ser phosphorylation site {S277} Ser phosphorylation site {S280} Ser phosphorylation site {S284} Ser phosphorylation site {S297} Ser phosphorylation site {S300} Ser phosphorylation site {S329} Ser phosphorylation site {S337} Ser phosphorylation site {S353} Thr phosphorylation site {T354} Ser phosphorylation site {S402} PDZ domain NAME: PDZ domain SITE: 421-502 src homology 3 [SH3] domain SITE: 516-584 domain {598-779} MOTIF: Ser phosphorylation site {S617} HOMOLOGY: guanylate kinase Thr phosphorylation site {T868} Ser phosphorylation site {S912} Ser phosphorylation site {S927} Ser phosphorylation site {S968} Tyr phosphorylation site {Y1140} Tyr phosphorylation site {Y1165} proline-rich region SITE: 1243-1248 MOTIF: proline residue (SEVERAL) Tyr phosphorylation site {Y1354} Ser phosphorylation site {S1366} proline-rich region SITE: 1426-1432 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S1545} Ser phosphorylation site {S1617} Ser phosphorylation site {S1619} ZU5 {1632-1724}

Database Correlations

OMIM 601009 UniProt Q07157 PFAM correlations Entrez Gene 7082 Kegg hsa:7082