RuvB-like 1 (49-kD TATA box-binding protein-interacting protein, 49 kD TBP-interacting protein, TIP49a, Pontin 52, nuclear matrix protein 238, NMP 238, 54 kD erythrocyte cytosolic protein, ECP-54, TIP60-associated protein 54-alpha, TAP54-alpha, RUVBL1, NMP238, TIP49, TIP49A)

Function: - possesses single-stranded DNA-stimulated ATPase & ATP-dependent DNA helicase (3' to 5') activity - component of the NuA4 histone acetyltransferase complex - NuA4 complex ATPase & helicase activities contributed by association of RUVBL1 & RUVBL2 with EP400 - RUVBL1 plays an essential role in oncogenic transformation by MYC & also modulates transcriptional activation by the LEF1/TCF1- CTNNB1 complex - may be able to bind plasminogen at cell surface & enhance plasminogen activation - essential for cell proliferation - forms homotypic & heterotypic interactions - forms a multimeric complex with RUVBL2 - interacts with the transcriptional activation domain of MYC - component of the RNA polymerase 2 holoenzyme complex - may also act to bridge the LEF1/TCF1-CTNNB1 complex & TBP - associates with alpha & gamma tubulins, particularly during metaphase & early anaphase - interacts with NPAT Structure: - binding to MYC is dependent on a Myc domain essential for oncogenic activity - belongs to the ruvB family Compartment: - nuclear matrix, nucleus, nucleoplasm cytoplasm, membrane - mainly localized in the nucleus - associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm & associated with the cell membranes - in prophase & prometaphase, it is located at the centrosome & the branching microtubule spindles - after mitotic nuclear membrane disintigration it accumulates at the centrosome & sites of tubulin polymerization - as cells pass through metaphase & into telophase it is located close to the centrosome at the early phase of tubulin polymerization - in anaphase, it accumulates at the zone of tubule interdigitation - in telophase, it is found at polar tubule overlap, & it reappears at the site of chromosomal decondensation in the daughter cells Alternative splicing: named isoforms=2 Expression: - ubiquitously expressed with high expression in heart, skeletal muscle & testis Pathology: - high level of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases including polymyositis/dermatomyosistis & autoimmune hepatitis

Properties

SIZE: MW = 50 kD entity length = 456 aa COMPARTMENT: cell nucleus MOTIF: ATP-binding site NAME: ATP-binding site SITE: 70-77

Database Correlations

OMIM 603449 UniProt Q9Y265 PFAM correlations Kegg hsa:8607

References

UniProt :accession Q9Y265