E3 ubiquitin-protein ligase TRIP12; thyroid receptor-interacting protein 12; TR-interacting protein 12; TRIP-12 (TRIP12, KIAA0045)

Function: - component of PA700, an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex - specifically interacts with the ligand binding domain of the thyroid hormone receptor (thyroid hormone T3-independent) & with retinoid X receptor (RXR) - could be E3 ubiquitin- protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester & then directly transfers the ubiquitin to targeted substrates - a Cys is required for ubiquitin-thioester formation - protein modification; protein ubiquitination - phosphorylated upon DNA damage, probably by ATM or ATR Structure: - belongs to the UPL family, K-HECT subfamily - contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain - contains 1 WWE domain

General

phosphoprotein protein subunit thyroid hormone receptor interacting protein (TRIP) E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein

Properties

SIZE: entity length = 1992 aa MW = 220 kD MOTIF: Ser phosphorylation site {S77} Ser phosphorylation site {S85} Ser phosphorylation site {S88} Ser phosphorylation site {S310} Ser phosphorylation site {S312} Tyr phosphorylation site {Y507} WWE {749-836} Ser phosphorylation site {S991} Ser phosphorylation site {S997} Ser phosphorylation site {S1317} Ser phosphorylation site {S1322} Ser phosphorylation site {S1427} K-box {1496-1570} Ser phosphorylation site {S1577} Thr phosphorylation site {T1592} HECT domain {1885-1992} MOTIF: cysteine residue {C1959}

Database Correlations

OMIM 604506 UniProt Q14669 Pfam PF00632 Entrez Gene 9320 Kegg hsa:9320

References

UniProt :accession Q14669