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transcription factor IIH (TFIIH) or BTF2

Function: 1) nucleotide excision repair (NER) of DNA 2) RNA transcription by RNA polymerase 2 (when complexed to CAK) 3) binds to TFIIF/RNA polymerase II/TFIID/TFIIA/TFIIB & is required for transcription 4) TFIIH contains: a) CDK7 as catalytic subunit of kinase activity at C-terminal domain [RNA polymerase-2 CTD consensus repeat] of RNA pol II L' subunit b) helicase activity for RNA polymerase II-mediated transcription 5) two of its subunits comprise protein products of genes that correct DNA repair deficits in Xeroderma Pigmentosum 6) TFIIH may function as an excision repair complex independent of its involvement in transcription. Structure: - components include: - ERCC2, ERCC3, GTF2H1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, MNAT1, CDK7, CCNH

General

general transcription factor (basal transcription factor, transcription initiation factor) molecular complex

Properties

COMPARTMENT: cell nucleus SUBUNITS: TFIIH basal transcription factor complex helicase XPD subunit MOTIF: DNA-binding motif MOTIF: helix-loop-helix NAME: helix-loop-helix FOR-BINDING-VIA: helix-loop-helix active site MOTIF: P-loop FOR-BINDING-OF: adenosine triphosphate binding site SITE: 1-248 FOR-BINDING-OF: general transcription factor IIH subunit 3 MOTIF: binding site SITE: 1-162 FOR-BINDING-OF: TFIIH basal transcription factor complex helicase XPB subunit DNA repair protein complementing XP-G cells general transcription factor IIH subunit 2 binding site SITE: 342-760 FOR-BINDING-OF: transcription factor IIH 62 kD subunit MOTIF: binding site SITE: 342-434 FOR-BINDING-OF: DNA repair protein complementing XP-G cells TFIIH basal transcription factor complex helicase XPB subunit MOTIF: nuclear translocation signal {6-18} acidic region {20-28} MOTIF: acidic residue (SEVERAL) acidic region {256-265} MOTIF: acidic residue (SEVERAL) helicase NAME: helicase SITE: 327-488 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 340-347 Ser phosphorylation site {S370} DEVH box {441-444} Helicase C-terminal {542-702} MOTIF: acidic region {697-700} MOTIF: acidic residue (SEVERAL) acidic region {721-728} MOTIF: acidic residue (SEVERAL) CDK-activating kinase STATE: active state MOTIF: S/T phosphorylation site kinase domain MOTIF: ATP-binding site NAME: ATP-binding site general transcription factor IIH subunit 3 MOTIF: Zn finger C4-type SITE: 268-285 EFFECTOR-BOUND: Zn+2 general transcription factor IIH subunit 2 MOTIF: VWFA domain {60-236} Zn finger C4-type SITE: 291-308 EFFECTOR-BOUND: Zn+2 general transcription factor IIH subunit 4 transcription factor IIH 62 kD subunit

References

  1. Ha I, Lane WS, Reinberg D. Cloning of a human gene encoding the general transcription initiation factor IIB. Nature. 1991 Aug 22;352(6337):689-95. PMID: 1876184
  2. Buratowski S. DNA repair and transcription: the helicase connection. Science. 1993 Apr 2;260(5104):37-8. No abstract available. PMID: 8465198
  3. Schaeffer L, Roy R, Humbert S, Moncollin V, Vermeulen W, Hoeijmakers JH, Chambon P, Egly JM. DNA repair helicase: a component of BTF2 (TFIIH) basic transcription factor. Science. 1993 Apr 2;260(5104):58-63. PMID: 8465201
  4. Drapkin R, Reinberg D. Transcription. The essential twist. Nature. 1994 Jun 16;369(6481):523-4. No abstract available. PMID: 8202151
  5. Drapkin R, Sancar A, Reinberg D. Where transcription meets repair. Cell. 1994 Apr 8;77(1):9-12. Review. PMID: 8156601
  6. Drapkin R, Reinberg D. The multifunctional TFIIH complex and transcriptional control. Trends Biochem Sci. 1994 Nov;19(11):504-8. Review. PMID: 7855896
  7. Shiekhattar R, Mermelstein F, Fisher RP, Drapkin R, Dynlacht B, Wessling HC, Morgan DO, Reinberg D. Cdk-activating kinase complex is a component of human transcription factor TFIIH. Nature. 1995 Mar 16;374(6519):283-7. PMID: 7533895
  8. Winkler GS, Vermeulen W, Coin F, Egly JM, Hoeijmakers JH, Weeda G. Affinity purification of human DNA repair/transcription factor TFIIH using epitope-tagged xeroderma pigmentosum B protein. J Biol Chem. 1998 Jan 9;273(2):1092-8. PMID: 9422774
  9. UniProt :accession Q6ZYL4

Component-of

RNA polymerase-2 yeast holoenzyme

Components

CDK7/cyclin H complex, CDK-activating kinase (CAK) or p34cdc2 activating kinase general transcription factor IIH subunit 2; basic transcription factor 2 44 kD subunit; BTF2 p44; general transcription factor IIH polypeptide 2; TFIIH basal transcription factor complex p44 subunit (GTF2H2 BTF2P44) general transcription factor IIH subunit 3; basic transcription factor 2 34 kD subunit; BTF2 p34; general transcription factor IIH polypeptide 3; TFIIH basal transcription factor complex p34 subunit (GTF2H3) general transcription factor IIH subunit 4; basic transcription factor 2 52 kD subunit; BTF2 p52; general transcription factor IIH polypeptide 4; TFIIH basal transcription factor complex p52 subunit; p52TFIIH (GTF2H4) TFIIH basal transcription factor complex helicase XPB subunit; basic transcription factor 2 89 kD subunit; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; TFIIH basal transcription factor complex 89 kD subunit; TFIIH 89 kD subunit; TFIIH p89; Xeroderma pigmentosum group B-complementing protein (ERCC3, XPB, XPBC, p89TFIIH) TFIIH basal transcription factor complex helicase XPD subunit; basic transcription factor 2 80 kD subunit; BTF2 p80; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; TFIIH basal transcription factor complex 80 kD subunit; TFIIH 80 kD subunit; TFIIH p80; Xeroderma pigmentosum group D-complementing protein (ERCC2, XPD, XPDC) transcription factor IIH 62 kD subunit; transcription factor B1 in yeast (p62TFIIH)