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transcription factor IIF (TFIIS)
Function:
- RNA polymerase 2 elongation factor
- inhibits premature termination of RNA polymerase 2 activity
- facilitates binding of RNA polymerase 2 to DAB commlex (TFIID/TFIIA/TFIIB)
- required for transcription.
Structure:
- heterodimer TFIIF-alpha & TFIIF-beta subunits [3,5,6]
- RNA Polymerase 2 recruitment activity resides in TFIIF-beta
General
general transcription factor (basal transcription factor, transcription initiation factor)
multisubunit protein
transcription elongation factor
Properties
COMPARTMENT: cell nucleus
SUBUNITS: general transcription factor IIF subunit 2
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
ATP-binding site
NAME: ATP-binding site
SITE: 36-43
Ser phosphorylation site {S142}
Ser phosphorylation site {S248}
general transcription factor IIF subunit 1
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
Ser phosphorylation site {S217}
Ser phosphorylation site {S218}
Ser phosphorylation site {S221}
Ser phosphorylation site {S224}
glutamate-rich region {283-350}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S305}
Ser phosphorylation site {S307}
Ser phosphorylation site {S308}
Ser phosphorylation site {S311}
Thr phosphorylation site {T331}
Ser phosphorylation site {S342}
Ser phosphorylation site {S345}
Ser phosphorylation site {S347}
Ser phosphorylation site {S348}
Ser phosphorylation site {S351}
Ser phosphorylation site {S355}
Ser phosphorylation site {S377}
Ser phosphorylation site {S380}
Ser phosphorylation site {S381}
Ser phosphorylation site {S385}
Thr phosphorylation site {T389}
Ser phosphorylation site {S391}
Ser phosphorylation site {S431}
Ser phosphorylation site {S433}
Ser phosphorylation site {S436}
Ser phosphorylation site {S442}
Thr phosphorylation site {T445}
References
- Molecular Cell Biology (2nd ed) Darnell J; Lodish H
& Baltimore D (eds), Scientific American Books,
WH Freeman, NY 1990, pg 294
- Ha I, Lane WS, Reinberg D.
Cloning of a human gene encoding the general transcription
initiation factor IIB.
Nature. 1991 Aug 22;352(6337):689-95.
PMID: 1876184
- Buratowski S.
The basics of basal transcription by RNA polymerase II.
Cell. 1994 Apr 8;77(1):1-3. Review. No abstract available.
PMID: 8156586
- Drapkin R, Reinberg D.
The multifunctional TFIIH complex and transcriptional control.
Trends Biochem Sci. 1994 Nov;19(11):504-8. Review.
PMID: 7855896
- Joliot V, Demma M, Prywes R.
Interaction with RAP74 subunit of TFIIF is required for
transcriptional activation by serum response factor.
Nature. 1995 Feb 16;373(6515):632-5.
PMID: 7854423
- UniProt :accession P13984
Components
general transcription factor IIF subunit 1; general transcription factor IIF 74 kD subunit; transcription initiation factor IIF subunit alpha; TFIIF-alpha; transcription initiation factor RAP74 (GTF2F1, RAP74)
general transcription factor IIF subunit 2; ATP-dependent helicase GTF2F2; general transcription factor IIF 30 kD subunit; transcription initiation factor IIF subunit beta; TFIIF-beta; transcription initiation factor RAP30 (GTF2F2, RAP30)