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transcription factor IIB; general transcription factor; S300-II (GTF2B, TF2B, TFIIB)
Function:
- general transcription factor
- required for transcription
- role in the activation of eukaryotic genes transcribed by RNA polymerase 2
- associates with TFIID-TFIIA (DA complex) to form TFIID-TFIIA-TFIIB (DAB-complex) which is then recognized by RNA polymerase 2
- interacts with the transcription elongation factor TCEA2
- interacts with HIV-1 Vpr
- interacts with GPBP1 (putative)
- in yeast, TFIIB associates with RNA polymerase 2, TFIIH, & the SRB subcomplex to form the RNA pol II holoenzyme
Cofactor: binds 1 Zn+2 per subunit
Structure:
- belongs to the TFIIB family
- contains 1 TFIIB-type Zn+2 finger
Compartment: nucleus
General
adenosine triphosphatase (ATPase)
general transcription factor (basal transcription factor, transcription initiation factor)
Properties
SIZE: entity length = 316 aa
MW = 35 kD
COMPARTMENT: cell nucleus
MOTIF: Zinc finger
NAME: Zinc finger
SITE: 11-42
EFFECTOR-BOUND: Zn+2
MOTIF: Zn+2-binding site
SITE: 15-15
Zn+2-binding site
SITE: 18-18
Zn+2-binding site
SITE: 34-34
Zn+2-binding site
SITE: 37-37
consensus repeat {124-200}
consensus repeat {218-294}
Database Correlations
OMIM 189963
UniProt Q00403
PFAM correlations
Kegg hsa:2959
References
- Molecular Cell Biology (2nd ed) Darnell J; Lodish H
& Baltimore D (eds), Scientific American Books,
WH Freeman, NY 1990, pg 294
- Ha I, Lane WS, Reinberg D.
Cloning of a human gene encoding the general transcription initiation factor IIB.
Nature. 1991 Aug 22;352(6337):689-95.
PMID: 1876184
- Buratowski S.
The basics of basal transcription by RNA polymerase II.
Cell. 1994 Apr 8;77(1):1-3. Review.
PMID: 8156586
- UniProt :accession Q00403
Component-of
RNA polymerase-2 yeast holoenzyme