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telomeric repeat-binding factor 1; NIMA-interacting protein 2; TTAGGG repeat-binding factor 1; Telomeric protein Pin2/TRF1 (TERF1, PIN2, TRBF1, TRF1)

Function: 1) involved in mitotic spindle checkpoint/mitotic regulation 2) binds the telomeric double-stranded TTAGGG repeat & negatively regulates telomere length 3) important for maintaining mitotic checkpoint in response to DNA damage 4) interacts with ATM, TINF2, TNKS1, TNKS2, PINX1, NEK2, MAPRE1 5) phosphorylated preferentially on Ser-219 by ATM kinase in response to ionizing DNA damage. 6) ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA 7) found in a complex with POT1, TINF2 & TNKS1. Structure: - homodimer - the acidic N-terminal domain binds to the ankyrin repeats of TNKS1 & TNKS2 - C-terminal domain binds microtubules Compartment: - nuclear - colocalizes with telomeric DNA in interphase & metaphase cells - located at chromosome ends during metaphase - associates with the mitotic spindle Expression: - highly expressed & ubiquitous - isoform Pin2 predominates - Pin2 expression is tighly regulated during the cell cycle - levels of Pin2 are low in G1 & S phase & increase during G2 phase & mitosis Notes: - Pin2 is identical to TRF1 except for a 20 amino acid deletion

Related

PIN2/TERF1-interacting telomerase inhibitor 1; liver-related putative tumor suppressor; Pin2-interacting protein X1; protein 67-11-3; TRF1-interacting protein 1 (PINX1 LPTL LPTS)

General

phosphoprotein peptidyl-prolyl cis/trans isomerase, NIMA-interacting (Pin) telomeric repeat binding factor

Properties

SIZE: entity length = 439 aa MW = 50 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: acidic region {2-71} MOTIF: acidic residue (SEVERAL) acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Ser phosphorylation site {S11} glutamate-rich region {55-62} MOTIF: glutamate residue (SEVERAL) TRFH dimerization {58-268} MOTIF: Ser phosphorylation site {S219} RLIM interaction {265-378} MOTIF: nuclear translocation signal {337-356} HTH myb-type {375-432} MOTIF: DNA-binding motif SITE: 403-428 Ser phosphorylation site {S434} Ser phosphorylation site {S435} Ser phosphorylation site {S437}

Database Correlations

OMIM 600951 UniProt P54274 PFAM correlations Entrez Gene 7013 Kegg hsa:7013

References

  1. Zhou XZ & Lu KP The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. Cell 107:347-59, 2001 PMID: 11701125
  2. UniProt :accession P54274

Component-of

shelterin complex (telosome)