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synaptojanin-1; synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1 (SYNJ1, KIAA0910)
Function:
- inositol 5-phosphatase
- role in clathrin- mediated endocytosis
- binds AMPH, SH3GL1, SH3GL2 & SH3GL3
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
Structure:
- binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats
- the C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 & GRB2
- belongs to the synaptojanin family
- in the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family
- contains 1 RRM domain (RNA recognition motif)
- contains 1 SAC domain
Compartment: cytoplasm (putative)
Alternative splicing: named isoforms=4
Expression:
- concentrated at clathrin-coated endocytic intermediates in nerve terminals
- isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells
- isoform 2 is very abundant in nerve terminals
General
phosphomonoesterase
phosphoprotein
RNA-binding protein
Properties
SIZE: entity length = 1573 aa
MW = 173 kD
COMPARTMENT: cytoplasm
MOTIF: Sac phosphatase domain {119-442}
active site
SITE: 500-899
MOTIF: Tyr phosphorylation site {Y784}
Tyr phosphorylation site {Y786}
Ser phosphorylation site {S830}
proline-rich region
SITE: 900-1573
MOTIF: proline residue (SEVERAL)
RNP motif
NAME: RNP motif
SITE: 902-971
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
NAME: ribonucleoprotein-1 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
ribonucleoprotein-2 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
serine-rich region {1033-1036}
MOTIF: serine residue (SEVERAL)
Thr phosphorylation site {T1048}
Ser phosphorylation site {S1053}
Ser phosphorylation site {S1084}
proline-rich region
SITE: 1108-1113
MOTIF: proline residue (SEVERAL)
proline-rich region
SITE: 1126-1129
MOTIF: proline residue (SEVERAL)
Ser phosphorylation site {S1150}
Thr phosphorylation site {T1220}
Ser phosphorylation site {S1345}
Thr phosphorylation site {T1349}
Ser phosphorylation site {S1392}
3 X 3 AA repeats of N-P-F {1396-1419}
consensus repeat {1396-1398}
consensus repeat {1406-1408}
consensus repeat {1417-1419}
glutamate-rich region {1485-1488}
MOTIF: glutamate residue (SEVERAL)
proline-rich region
SITE: 1538-1544
MOTIF: proline residue (SEVERAL)
Ser phosphorylation site {S1551}
Ser phosphorylation site {S1565}
Database Correlations
OMIM 604297
UniProt O43426
PFAM correlations
Entrez Gene 8867
Kegg hsa:8867
ENZYME 3.1.3.36
References
UniProt :accession O43426