Symplekin (SYMPK SPK)

Function: - heat-labile component of a multimolecular complex that functiond in histone mRNA 3'-end processing - specific component of the tight junction (TJ) plaque - might not be an exclusively junctional component - may have a house-keeping rule - may be required for pre-mRNA polyadenylation - found in a heat-sensitive complex at least composed of several cleavage & polyadenylation specific & cleavage stimulation factors - interacts with CPSF2, CPSF3 & CSTF2 - interacts with HSF1 in heat-stressed cells Compartment: - cytoplasm, cytoskeleton - cell junction, tight junction - cell membrane; peripheral membrane protein, cytoplasmic side - nucleus, nucleoplasm - cytoplasmic face of adhesion plaques (major) & nucleoplasm (minor) (in cells with tight junction) - nucleoplasm (in cells without tight junction) - nuclear bodies of heat-stressed cells Alternative splicing: - named isoforms=3 - additional isoforms seem to exist Expression: - expressed in testis - expressed in polar epithelia & Sertoli cells - not expressed in vascular endothelia - detected in stomach, duodenum, pancreas, liver, fetal brain, carcinomas, lens-forming cells, fibroblasts, lymphocytes, lymphoma cells, erythroleukemia cells (at the protein level) - not found in endothelium of vessels, epidermis, intercalated disks, purkinje fiber cells of the heart & lymph node Pathology: - may see use as a differentiation marker in the differential diagnosis of tumors

Properties

SIZE: entity length = 1274 aa MW = 141 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: HSF1 interaction {2-124} acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl nuclear translocation signal {345-360} Ser phosphorylation site {S494} serine-rich region {1168-1175} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S1171} Ser phosphorylation site {S1175} Ser phosphorylation site {S1243} Thr phosphorylation site {T1257} Ser phosphorylation site {S1259}

Database Correlations

OMIM 602388 UniProt Q92797 PFAM correlations Entrez Gene 8189 Kegg hsa:8189

References

UniProt :accession Q92797