transcription elongation factor SPT5; hSPT5; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; Tat-cotransactivator 1 protein; Tat-CT1 protein (SUPT5H, SPT5, SPT5H)

Function: - component of the DSIF complex - component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 & CCNT1, RNA polymerase 2, SUPT5H, & NCL/nucleolin - methylated by PRMT1/HRMT1L2 & PRMT5/SKB1 - methylation negatively regulates interaction with P-TEFb & RNA polymerase 2 - phosphorylation by P-TEFb alleviates transcriptional pausing & can stimulate transcriptional elongation from the HIV-1 LTR - P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein - phosphorylation may also stimulate interaction with PIN1 - bulk phosphorylation occurs predominantly in mitosis Structure: - belongs to the SPT5 family - contains 5 KOW domains Compartment: nucleus Alternative splicing: named isoforms=2 Expression: ubiquitously expressed

General

phosphoprotein transcription elongation factor

Properties

SIZE: MW = 121 kD entity length = 1087 aa COMPARTMENT: cell nucleus MOTIF: glutamate-rich region {11-106} MOTIF: glutamate residue (SEVERAL) binding site SITE: 176-270 FOR-BINDING-OF: transcription elongation factor SPT4 KOW 1 {273-306} RNA pol-2 interaction {313-420} KOW 2 {420-451} KOW 3 {472-503} KOW 4 {594-627} Ser phosphorylation site {S666} KOW 5 {704-737} MOTIF: Thr phosphorylation site {T709} Tyr phosphorylation site {Y717} CTR1 {754-817} MOTIF: G- S-[QR]-T-P-X-[YQ] {754-817} (9) Thr phosphorylation site {T775} Thr phosphorylation site {T784} CTR2 NAME: CTR2 SITE: 844-968 MOTIF: proline residue (SEVERAL) P-[TS]-P-S-P-[QA]-[SG]-Y (10) Thr phosphorylation site {T1034}

Database Correlations

OMIM 602102 UniProt O00267 PFAM correlations

References

UniProt :accession O00267