signal transducing adapter molecule 2; STAM-2; Hrs-binding protein (STAM2, HBP)

Function: - role in intracellular signal transduction mediated by cytokines & growth factors - upon IL-2 & GM-CSL stimulation, plays a role in signaling leading to DNA synthesis & MYC induction - may also play a role in T-cell development - role in down-regulation of receptor tyrosine kinase via multivesicular body when complexed with HGS (ESCRT-0 complex) - phosphorylated in response to IL-2, GM-CSF, EGF & PDGF - component of the ESCRT-0 complex - interacts with JAK2 & JAK3 - interacts with ubiquitinated proteins & the deubiquitinating enzyme USP8/UBPY (putative) - interacts (via the via the PxVxL motif) with CBX5 - interacts with VPS37C - interacts with ubiquitin Structure: - the VHS & UIM domains mediate the interaction with ubiquitinated proteins - the SH3 domain mediates the interaction with USP8 - contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains - this motif requires additional residues -7, -6, +4 & +5 of the central Val which contact the chromoshadow domain - belongs to the STAM family - contains 1 ITAM domain - contains 1 SH3 domain - contains 1 UIM (ubiquitin-interacting motif) repeat - contains 1 VHS domain Compartment: - cytoplasm (putative) - early endosome membrane - peripheral membrane, cytoplasmic side Alternative splicing: named isoforms=2 Expression: ubiquitously expressed

Properties

SIZE: entity length = 525 aa MW = 58 kD COMPARTMENT: cytoplasm MOTIF: VHS domain {16-144} MOTIF: PxVxL {54-67} UIM {165-184} Tyr phosphorylation site {Y192} src homology 3 [SH3] domain SITE: 202-261 MOTIF: USP8 interaction {219-220} Tyr phosphorylation site {Y291} HGS interaction {334-368} ITAM {360-377} MOTIF: Tyr phosphorylation site {Y371} Tyr phosphorylation site {Y374}

Database Correlations

OMIM 606244 UniProt O75886 PFAM correlations Entrez Gene 10254 Kegg hsa:10254

References

UniProt :accession O75886